FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_HBEpC_LOW_2DPI_H1N1_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_HBEpC_LOW_2DPI_H1N1_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2659757
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT102280.38454640781093913No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA97060.36492055477248486No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC69680.2619788198696347No Hit
CTATTGGGACTCATCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTT67540.25393297207226073No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA64050.2408114726270107No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG62880.2364125745321847No Hit
GGGCTAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC62260.23408153451612307No Hit
ATGTAGGACCATGAACTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC61510.23126172804508083No Hit
TCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG59920.22528373832647117No Hit
CCTTAGCCCCATGGAACGTTATTTCTCTTTTAAGCTTCTTGTATAGTTTA57080.2146060711561244No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT56020.2106207446770513No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC55360.20813931498253413No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC55300.20791373046485073No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTTAAAAGAGAAATAACGTTCC53410.2008078181578242No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC53310.20043184396168523No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC52390.1969728813572067No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACTTGGAGAAAAGAATAGAGA49990.1879495006498714No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT48590.1826858619039258No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG48470.18223469286855906No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC48240.18136995221743943No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC48070.18073079608400316No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGCAG47450.17839975606794156No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA46700.17557994959689927No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC46320.1741512476515712No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC45400.17069228504709266No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT43630.16403754177543287No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAT42520.1598642281982903No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC42490.15975143593944863No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA42210.1586987081902595No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT42040.15805955205682323No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA41670.15666844753110903No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC41660.15663085011149513No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG40310.15155519846361903No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGATAAAGC40250.15132961394593566No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG39670.14914896360832963No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC39380.1480586384395266No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT39220.14745707972570427No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT38970.14651714423535683No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG38900.14625396229805956No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC38360.1442237016389091No Hit
GCACTGGAACTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG38060.1430957790504922No Hit
GAACTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGT37810.14215584356014477No Hit
TTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACC37600.14136629774825293No Hit
GTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACT37550.14117831065018344No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG37020.1391856474106469No Hit
ATACAAGAAGCTTAAAAGAGAAATAACGTTCCATGGGGCTAAGGAGGTGT36910.13877207579489403No Hit
GCATGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCTGCTGC36370.1367418151357436No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA35890.13493713899427653No Hit
GATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGC35790.1345611647981376No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC35560.13369642414701796No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT35240.13249330671937323No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT34520.12978629250717266No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT34480.12963590282871706No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA34130.12831999314223066No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC33890.12741765507149713No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGG33540.12610174538501073No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG33310.1252370047338911No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT33090.12440986150238535No Hit
GTTCACAAGTGGCACACACTAGACCAAAAGCAGCTTCTGTGGTCACTGTT32640.12271797761976No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC32620.12264278278053221No Hit
GGCTATGGAACAGGTGGCTGGATCGAGTGAACAGGCAGCAGAGGCCATGG32490.12215401632555153No Hit
CTATACAAGAAGCTTAAAAGAGAAATAACGTTCCATGGGGCTAAGGAGGT32430.12192843180786817No Hit
GTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCA31880.11986057372910383No Hit
GATTAGTGGATTGGTGGTAGCAGCCATTTGTCTGTGAGACCGATGCTGTG31750.11937180727412315No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT31610.11884544339952861No Hit
CCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGACGAGAG31600.1188078459799147No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA31450.11824388468570624No Hit
GCCTAGTCTGATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGAT30790.11576245499118905No Hit
GATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACC29900.11241628464555221No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA29830.11215310270825493No Hit
ATGTAGGATTGGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCA29770.11192751819057153No Hit
CAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGTGTG29540.11106277753945192No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG29530.11102518011983803No Hit
ATCTACTACTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG29260.11001004979026278No Hit
GCTTAGTGACACCTCCTTAGCCCCATGGAACGTTATTTCTCTTTTAAGCT28760.10813017880956795No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG28230.1061375155700314No Hit
CATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAG27880.104821605883545No Hit
TTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCC27840.10467121620508942No Hit
CAGTTAAACTATACAAGAAGCTTAAAAGAGAAATAACGTTCCATGGGGCT27550.10358089103628638No Hit
CCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAG27450.10320491684014743No Hit
GCTTAAAAGAGAAATAACGTTCCATGGGGCTAAGGAGGTGTCACTAAGCT27250.10245296844786948No Hit
CTCATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCT27220.1023401761890278No Hit
CCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTCTCAG27210.1023025787694139No Hit
ATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGT26950.10132504585945257No Hit
TCTCAGACCAGCACTGGAACTAGGATGAGTCCCAATAGTTCTCATTGCAT26860.1009866690829275No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT26760.10061069488678855No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG26600.10000913617296617No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCGC100.00709183144.99788145
GAGCGAA2550.0113.7473451
GTCACCG352.7632632E-7103.56991145
AGGCTCG2400.099.686035145
AGCGAGC2900.089.99869145
GGATTAA1250.086.998726145
ATGTGCG353.4385783E-582.85593145
TAAATTA1950.081.79368145
GCCCACC2000.076.12388145
GCGAAAG8800.075.8071752
CGAAAGC8900.075.7701343
AACTGCG300.001936209472.49894145
GCAGGTA23600.072.1822369
GAGCAAA42050.070.358351
TCTCCGC1250.069.59898145
ACTAGGT651.3990939E-766.9221145
GAGCCCC2150.064.068825145
GTAGAAA10400.063.449691
AGTATAG350.003564970862.1524662
ACGGACT350.003564970862.1524664
Produced by FastQC (version 0.11.5)