FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_HBEpC_LOW_1DPI_H3N2_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_HBEpC_LOW_1DPI_H3N2_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419250
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGGGTCGATAGACGCATTTGAAAAAAAGACG64720.4560155011449709No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG46450.3272855381363396No Hit
CTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC46440.32721507838647174No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA45990.3240443896424168No Hit
ACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCTGCTGT44570.31403910516117667No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC41960.2956491104456579No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG39260.2766249779813282No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA36340.2560507310199049No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA33720.23759027655451825No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA31970.22525982032763786No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT31240.22011625858728204No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT31120.21927074158886736No Hit
CATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCA30720.21645235159415185No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG29540.20813810110974104No Hit
GTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCCTGCACCATTCG29360.20686982561211906No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC28580.20137396512242384No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG27880.19644178263167164No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA27780.1957371851329928No Hit
CTCCCAGCACAGGTCTCATAGGCAAATGGTGGCAACAACCAACCCATTAA26530.18692971639950678No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTAT26400.18601373965122422No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG25770.1815747754095473No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT24880.17530385767130527No Hit
CTCCAACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCT24610.1734014444248723No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG23330.16438259644178263No Hit
CCCTTACCCTGGGTCTAGATCCGATATTCGGGATTACAGTTTGTTGGCTT22650.15959133345076626No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG22540.1588162762022195No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGGTCAAGTGAGCAGGCA21640.15247489871410957No Hit
TCCTAGCTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA20550.14479478597850978No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGGATGCGA20400.14373788973049145No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT20080.14148317773471905No Hit
CCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTGTCATTGGGAATG19930.14042628148670072No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA19430.13690329399330634No Hit
GCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTGGGGT19320.13612823674475955No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC19280.135846397745288No Hit
GTGCTGGGAGTCAGCAATCTGCTCACATGTTGCACATACCAGGCCAAATG18980.13373260524925135No Hit
GGGTAAGGGATATCCCCAGCAGAATAAGCATCTATTGGACAATAGTAAAA18940.1334507662497798No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG18900.13316892725030827No Hit
CTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAG18860.13288708825083673No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG17950.1264752510128589No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTTGCTTT17770.1252069755152369No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC17660.12443191826669016No Hit
ATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCTAC17620.1241500792672186No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC17600.12400915976748282No Hit
CTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAAGAAATGGGACCT17570.12379778051787917No Hit
TACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATTGTATA17560.12372732076801128No Hit
CTCATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCT17330.12210674652104986No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT17290.1218249075215783No Hit
GTCCAATAGATGCTTATTCTGCTGGGGATATCCCTTACCCTGGGTCTAGA17270.12168398802184252No Hit
TCATAAGGGTAACAGTTGCTGTGGGCTTTGCTGCGTTCAACAAAAAGGTC17210.12126122952263518No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA16870.118865598027127No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTGGGG16820.11851329927778757No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA16780.118231460278316No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT16740.11794962127884445No Hit
CCCTTATGATGTGCCGGATTATGCCTCCCTTAGGTCACTAGTTGCCTCAT16740.11794962127884445No Hit
CTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAA16290.1147789325347895No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC16120.11358111678703542No Hit
GTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCCATTGAGGGCA16040.1130174387880923No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT16030.11294697903822441No Hit
GACTAAGGGGATTTTGGGGTTTGTGTTCACGCTCACCGTGCCCAGTGAGC15660.11033996829311256No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCATGGAACGTTATCTCCCTCTT15490.10914215254535847No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA15330.10801479654747226No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACCGTCGT15330.10801479654747226No Hit
ATCCCAATGATACTCGCGGCAACAACAAGCGGGTCACTTGAATCGTTGCA15320.10794433679760437No Hit
GTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATAGGCAA15020.10583054430156774No Hit
ATCTATTAGTGTGCAGTTTTCTCCATCAAGGATCTGATGAGGACTGTCGC14990.10561916505196407No Hit
CATGAGAACAGAATGGTTTTGGCCAGCACTACAGCTAAGGCTATGGAGCA14950.10533732605249252No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT14820.10442134930420997No Hit
TAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCTAATT14660.10329399330632376No Hit
CTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAATGGGTTG14610.10294169455698432No Hit
CTCTTAGACCAGTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCC14600.10287123480711644No Hit
CTATTAACCATGAAGACTATCTTTGCTTTGAGCTACATTCTATGTCTGGT14540.1024484763079091No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA14520.10230755680817334No Hit
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC14290.10068698256121192No Hit
GATATTGAGAGGGTCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCGT14220.1001937643121367No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGCG100.0070908526145.0022145
ACGGACG100.0070908526145.0022145
AGGCTCG850.0102.354485145
ACCGGAG259.383062E-487.010513
GTCAAGG502.2691893E-887.001305145
CCGATAG300.001934982672.508764
GTCCACG300.001934982672.508763
GAGTCGC809.822543E-1172.5011145
GCAGGGG4650.068.5886849
AGGGTGT5350.066.409896
CATCGAT451.1933166E-464.4522254
AGCTGGG808.089046E-963.43846145
GCAGGGT5150.060.52219
CACGAGC651.093782E-555.770073145
TAGAAAC7300.055.6231582
CGCTTCT2650.054.723596
CCACGCT400.006048514554.381575
GTGGGGC400.006051044454.375824145
CGAAAGC4550.054.1823653
CCGCTTC2700.053.710195
Produced by FastQC (version 0.11.5)