FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_HBEpC_LOW_12HPI_H3N2_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_HBEpC_LOW_12HPI_H3N2_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086851
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGGGTCGATAGACGCATTTGAAAAAAAGACG120230.5761312139678396No Hit
CTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC94340.45206869105652486No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG83950.4022807569874418No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC83150.3984472298213912No Hit
ACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCTGCTGT79930.3830172829780373No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA78730.37726699222896126No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG71610.3431486004511103No Hit
GTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCCTGCACCATTCG68390.32771865360775637No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA63910.30625090147787265No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA62160.2978650608021368No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT56490.2706949370127527No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG55490.2659030280551894No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG55430.2656155135177356No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC52510.25162313936165065No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG52370.2509522721075918No Hit
CTCCCAGCACAGGTCTCATAGGCAAATGGTGGCAACAACCAACCCATTAA49490.23715157430980938No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTAT49350.23648070705575053No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA48490.23235966535224603No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT47020.22531555918462792No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG46090.22085908385409406No Hit
CTCCAACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCT43180.20691462878758474No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC41610.1993913317242103No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT41280.1978100017682144No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGGTCAAGTGAGCAGGCA40690.19498277548325205No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA40350.1933535264376805No Hit
GTGCTGGGAGTCAGCAATCTGCTCACATGTTGCACATACCAGGCCAAATG37550.17993618135650316No Hit
TCCTAGCTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA37390.17916947592329305No Hit
GCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTGGGGT36600.175383867846818No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA36350.17418589060742717No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC34840.16695010808150654No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT34590.1657521308421157No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT33130.15875594376407323No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG33130.15875594376407323No Hit
CTCATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCT32640.1564079083748672No Hit
TACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATTGTATA32370.1551140929563251No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTGGGG32370.1551140929563251No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC31170.1493638022072491No Hit
CTCTTAGACCAGTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCC31170.1493638022072491No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCATGGAACGTTATCTCCCTCTT30620.14672825228058928No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA30200.14471565051841268No Hit
GTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCCATTGAGGGCA30010.14380518781647564No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC29940.14346975418944619No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT29520.1414571524272696No Hit
CTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAA29460.14116963788981582No Hit
GACTAAGGGGATTTTGGGGTTTGTGTTCACGCTCACCGTGCCCAGTGAGC29260.14021125609830315No Hit
CATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCA29160.1397320652025468No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACCGTCGT28140.1348443180658322No Hit
CTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAATGGGTTG27810.1332629881098363No Hit
ATCCCAATGATACTCGCGGCAACAACAAGCGGGTCACTTGAATCGTTGCA27680.13264003994535306No Hit
CATGAGAACAGAATGGTTTTGGCCAGCACTACAGCTAAGGCTATGGAGCA26770.12827940279397043No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA26550.1272251828233065No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGGGT26430.1266501537483989No Hit
GTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATAGGCAA26300.12602720558391567No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG25940.12430211835919287No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACC25820.12372708928428527No Hit
CCACAATATCAAGTGCAAGATCCCAATGATACTCGCGGCAACAACAAGCG25680.1230562220302264No Hit
GTCCAAAATGCCCTCAATGGGAATGGAGATCCAAATAACATGGACAAAGC25540.12238535477616755No Hit
ATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTGCA24680.11826431307266307No Hit
GCACATACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATT24640.11807263671436055No Hit
CTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGG24530.11754552672902857No Hit
CCCTTACCCTGGGTCTAGATCCGATATTCGGGATTACAGTTTGTTGGCTT24510.11744968854987729No Hit
GGCAATGAGAGCCGTTGGGACTCATCCTAGCTCCAGTACTGGTCTAAGAG24500.11740176946030166No Hit
CCTCAAAGCCGAGATCGCGCAGAGACTTGAAGATGTCTTTGCTGGGAAAA24470.11725801219157478No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG24040.11519749133982254No Hit
GATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACC23910.11457454317533931No Hit
CTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTG23810.11409535227958298No Hit
TGTTTGAAGGGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA23770.11390367592128044No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC23400.11213066960698201No Hit
GTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAATG23260.11145980235292313No Hit
TCTGATAGGTCTGCAAATTTTCAAGAAGATCATCTCTTAGACCAGTACTG23160.1109806114571668No Hit
CCTCTGACTAAGGGGATTTTGGGGTTTGTGTTCACGCTCACCGTGCCCAG23120.11078893509886428No Hit
GACCAATTCTGTCACCTCTGACTAAGGGGATTTTGGGGTTTGTGTTCACG22990.11016598693438104No Hit
ATCAAGTGCAAGATCCCAATGATACTCGCGGCAACAACAAGCGGGTCACT22950.1099743105760785No Hit
AACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAAC22880.10963887694904906No Hit
TCTTAGACCAGTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCCT22690.10872841424711203No Hit
GGGTAAGGGATATCCCCAGCAGAATAAGCATCTATTGGACAATAGTAAAA22590.1082492233513557No Hit
GTTCTCATGTTTTATTAATGGGTTGGTTGTTGCCACCATTTGCCTATGAG22550.10805754699305316No Hit
GTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAA22540.10800962790347754No Hit
AGCTAAGGCTATGGAGCAAATGGCTGGGTCAAGTGAGCAGGCAGCGGAGG22520.10791378972432628No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA22440.10753043700772119No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA22360.10714708429111613No Hit
TATGAGACCTGTGCTGGGAGTCAGCAATCTGCTCACATGTTGCACATACC22210.10642829794748163No Hit
CTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAGCTCTCAGTTA21940.10513448252893953No Hit
CCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTGTCATTGGGAATG21690.1039365052895487No Hit
CAATGGGAATGGAGATCCAAATAACATGGACAAAGCAGTTAAACTGTATA21690.1039365052895487No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGGATGCGA21310.10211557988567463No Hit
GCTTTGTCCATGTTATTTGGATCTCCATTCCCATTGAGGGCATTTTGGAC21300.102067660796099No Hit
CACTTGACCCAGCCATTTGCTCCATAGCCTTAGCTGTAGTGCTGGCCAAA21190.10154055081076703No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG21110.10115719809416197No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG301.1021075E-7120.833755145
TAGTGCG259.3880587E-487.0003145
AGGCTCG1950.074.35923145
CGCTATC300.001934907372.510684
GTACCGG350.003563950862.156481
AACGCTA350.003564966162.1520082
ACGCTAT350.003564966162.1520083
AGGGTGT7950.058.3719756
CGAAAGC5850.058.256443
TCTCCGC1900.053.421238145
CAGTACG1500.053.1617559
CATAATA4050.050.1308362
CGAGACA1457.2759576E-1250.00017145
GCAGGGG6750.049.4028439
TAGAAAC12750.048.909163
GCAGGGT9050.048.8628929
CGTTAAA450.00964046148.3335145
GACTGCC450.00964046148.3335145
AGCGAAA7200.047.336761
ACAAGGT11100.046.3807033
Produced by FastQC (version 0.11.5)