FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_HBEpC_HIGH_6HPI_H3N2_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_HBEpC_HIGH_6HPI_H3N2_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2049724
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC94610.4615743387890272No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT79960.3901013014435114No Hit
CTTTTAAGGCCGTGTTTGAAGGGTCGATAGACGCATTTGAAAAAAAGACG64100.3127250302967619No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA61660.3008209885818774No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA59320.28940481742907825No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC52510.25618083215106036No Hit
ACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCTGCTGT48960.23886142719702752No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT46550.22710374665076857No Hit
CTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC45980.22432288444688164No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG44320.21622423311626346No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC43110.21032099931502973No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG42980.20968676758431867No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA41980.20480806196346435No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC40910.19958784694915022No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT37330.18212208082649176No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG37000.18051210797160983No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC36290.17704822698080327No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC36230.176755504643552No Hit
ATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTCAAC33760.16470510176004183No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA33220.1620706007247805No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA32270.1574358303849689No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA31820.15524041285558446No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA31530.1538255882255367No Hit
GTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCCTGCACCATTCG31170.15206925420202913No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT29980.1462635945132125No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT29330.1430924358596572No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC29030.1416288241734009No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG28180.13748192439567475No Hit
GTTCTTATCTCCTGTTCTACTTCAAACAGCAGTTGTAATGCTTGCATGAA28070.13694526677738075No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG27760.13543286803491594No Hit
CCATAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA27680.13504257158524757No Hit
CCCTAAGAGGAAGAGGCAATACTCTCGGTCTAGACATCAAAGCAGCCACC27390.13362774695519983No Hit
GACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCT27100.13221292232515208No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG26790.13070052358268724No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTAT26480.12918812484022238No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA26470.12913933778401385No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG26180.1277245131539661No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT26150.12757815198534045No Hit
ATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATTGC26000.12684634614221232No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGAACT25780.12577303090562436No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA25580.12479728978145349No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT25290.12338246515140575No Hit
ACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGCTA25080.12235793697102636No Hit
CTCCCAGCACAGGTCTCATAGGCAAATGGTGGCAACAACCAACCCATTAA24890.12143098290306402No Hit
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC24790.12094311234097858No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG24660.12030888061026752No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT24420.1191379912612625No Hit
CTATTGAGGAATTGTCAAGAAACTGGTTCATGCTAATGCCCAAGCAGAAA24350.1187964818678027No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA24250.11830861130571727No Hit
CTCCAACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCT24180.11796710191225745No Hit
CTCCTGTTCTACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT23850.11635712905737554No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT23660.11543017498941321No Hit
CATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCA23530.11479594325870214No Hit
GTCTAGACATCAAAGCAGCCACCCATGTTGGAAAGCAAATTGTAGAAAAG22970.11206386811102372No Hit
CAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATT22870.1115759975489383No Hit
CTTATCTCCTGTTCTACTTCAAACAGCAGTTGTAATGCTTGCATGAATGT22490.10972208941301366No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGGTCAAGTGAGCAGGCA22240.10850241300780006No Hit
ATACTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTT21950.10708758837775231No Hit
ACCAAGAACTGAGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAG21880.10674607898429252No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT21840.10655093075945836No Hit
CTTAAAATGACCATGGTCTCCACACCTGCTTCGCGATACATAACTGACAT21760.10616063430979No Hit
GTTTTTAGTCTGTGTCTCACTTCTTCAATCAGCCATCTTATCTCTTCAAA21640.10557518963528749No Hit
GTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT21560.10518489318561913No Hit
CTCCACACCTGCTTCGCGATACATAACTGACATGACTATTGAGGAATTGT21350.10416036500523972No Hit
TAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGCTAATG21100.10294068860002616No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA20880.10186737336343821No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT20650.10074527107064171No Hit
ACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTCACCAT20590.10045254873339043No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGCC100.0070935525144.985299
CGCCCGG203.864826E-4108.762851
CCTAGCG259.389218E-486.997536145
CCTATCG406.6617606E-572.50685
GCAGGGG4550.070.102789
CACGAGC554.063806E-665.90723145
GCAGGGT13850.065.4265759
GTAAGAC350.00356536962.1501961
CCACGAC702.336219E-762.1411145
CAGTACG4400.060.959729
GAGTCGC851.3065801E-859.704197145
TAGAAAC14100.055.537122
CCGGCCT400.006049545554.3800964
TCACGCA400.00605246554.373463145
GCCCACC1600.054.373463145
ATGTGCG400.00605246554.373463145
GTTCGGG400.00605246554.373463145
CGGCTCA400.00605246554.373463145
CGAAAGC8800.053.5561563
AGCAGGG23300.053.5138858
Produced by FastQC (version 0.11.5)