FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_HBEpC_HIGH_6HPI_H3N2_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_HBEpC_HIGH_6HPI_H3N2_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727989
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC90130.5215889684482946No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT69520.40231737586292504No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA54480.3152797847671484No Hit
CTTTTAAGGCCGTGTTTGAAGGGTCGATAGACGCATTTGAAAAAAAGACG51230.2964717946699892No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG48780.2822934636736692No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC46330.2681151326773492No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA45630.26406418096411494No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG44820.25937665112451525No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA42680.24699231302977045No Hit
ACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCTGCTGT38120.2206032561549871No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC36740.21261709420603953No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT35870.20758233993387687No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG34900.20196887827410936No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC34760.20115868793146252No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT34530.19982766093997129No Hit
CTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC33580.19432994075772472No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT33430.1934618796763174No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC31430.18188773192421942No Hit
ATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTCAAC30610.17714233134585927No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT30450.17621639952569143No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA29690.1718182233798942No Hit
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC28780.16655198615268962No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC28380.16423715660227003No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA27900.16145936114176654No Hit
GTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCCTGCACCATTCG26870.1554986750494361No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT26660.1542833895354658No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC25850.1495958596958661No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG25690.14866992787569827No Hit
CCATAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA25000.14467684690122448No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA24930.14427175172990106No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA24740.14317220769345174No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTAT24630.14253562956708637No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG24260.14039441223294824No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG24080.13935273893525943No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA23270.13466520909565977No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA22980.13298695767160557No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT22630.1309614818149884No Hit
CCCTAAGAGGAAGAGGCAATACTCTCGGTCTAGACATCAAAGCAGCCACC22590.13072999885994646No Hit
GACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCT22450.1299198085172996No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGAACT22080.12777859118316146No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA21990.12725775453431706No Hit
ACTAGAGACCATAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTG21980.12719988379555658No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG21770.12598459828158629No Hit
ATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATTGC21730.12575311532654432No Hit
ACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGCTA21610.12505866646141844No Hit
CTATTGAGGAATTGTCAAGAAACTGGTTCATGCTAATGCCCAAGCAGAAA21280.12314893208232229No Hit
CTCCCAGCACAGGTCTCATAGGCAAATGGTGGCAACAACCAACCCATTAA21090.12204938804587298No Hit
CTCCAACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCT20830.12054474883810024No Hit
GTCTAGACATCAAAGCAGCCACCCATGTTGGAAAGCAAATTGTAGAAAAG20670.11961881701793241No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT20530.11880862667528555No Hit
ACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTCACCAT20440.11828779002644113No Hit
GTTCTTATCTCCTGTTCTACTTCAAACAGCAGTTGTAATGCTTGCATGAA20340.11770908263883624No Hit
GTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT19970.1155678653046981No Hit
CAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATT19730.11417896757444637No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT19470.11267432836667363No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT19450.11255858688915266No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG18890.10931782551856523No Hit
CATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCA18820.10891273034724178No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA18610.1076974448332715No Hit
CTCCTGTTCTACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT18570.10746596187822956No Hit
ACCAAGAACTGAGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAG18510.1071187374456666No Hit
CTTATCTCCTGTTCTACTTCAAACAGCAGTTGTAATGCTTGCATGAATGT18490.10700299596814564No Hit
CTCCACACCTGCTTCGCGATACATAACTGACATGACTATTGAGGAATTGT18350.10619280562549878No Hit
GTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTCAACATGATGTT17820.10312565647119282No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGGTCAAGTGAGCAGGCA17640.10208398317350399No Hit
CTTAAAATGACCATGGTCTCCACACCTGCTTCGCGATACATAACTGACAT17520.10138953430837812No Hit
GTGCTGGGAGTCAGCAATCTGCTCACATGTTGCACATACCAGGCCAAATG17460.1010423098758152No Hit
TAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGCTAATG17300.10011637805564734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGTG100.007090929145.002583
AGGCTCG1150.0119.770874145
TAGTGCG259.391568E-486.99148145
CGGCTCG353.439658E-582.84902145
TATAGTG809.822543E-1172.501295
CTGCCCG300.001936734472.49289145
CGGTCGA300.001936734472.49289145
GATATAG851.6916601E-1068.23653
GCTTATA2900.067.50122
CACGAGC554.0652176E-665.90263145
GCACGGG451.1946826E-464.438126145
GCAGGGG4450.063.5275849
ACACTCG1150.063.0445984
ATGCGGG350.00356832862.13677145
CGAAAGC6650.061.053723
AGCGAAA6550.059.7755171
CGGGAGC502.0116298E-457.994316145
CCACGAC753.7653626E-757.994316145
AGCTTAT3600.054.3791121
GCACTAA400.006049817454.3791121
Produced by FastQC (version 0.11.5)