FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_HBEpC_HIGH_6HPI_H1N1_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_HBEpC_HIGH_6HPI_H1N1_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600757
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC19320.32159425524796215No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG15520.25834072678304204No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA14730.24519065112849286No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT13410.2232183728196259No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT13290.22122089297336528No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT12510.20823727397267117No Hit
TTCCTTGACCGGCTACGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCAA9800.1631275207779518No Hit
TTCTAAGTGTCTCGCTGGATTCCTCTTTCAAAATCCATTCCACGATTTGT9440.15713508123916992No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATCGTG9230.1536394915082138No Hit
GGATTGGGTGATGCCCCATTCCTTGACCGGCTACGCCGAGATCAAAAGTC9110.15164201166195315No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG9100.15147555500809812No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC8800.1464818553924465No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT8220.13682736946885346No Hit
GTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAA8090.13466343296873778No Hit
GGCATAAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA7880.13116784323778166No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACTTGGAGAAAAGAATAGAGA7560.12584123031442No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC7330.12201272727575375No Hit
TCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG7290.12134690066033355No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCTCGCCACTTCTCATTTC7110.1183506808909426No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC7050.11735194096781229No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT7000.11651965769853702No Hit
CTATTGGGACTCATCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTT6960.11585383108311681No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC6840.11385635123685617No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT6710.11169241473674048No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTTATG6600.1098613915443349No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT6490.10803036835192932No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG6450.10736454173650911No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA6420.10686517177494394No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAATGTAA6230.10370249535169794No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA6140.10220438546700247No Hit
ATGTAGGACCATGAACTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC6080.10120564554387215No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT6050.10070627558230699No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG6050.10070627558230699No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT6010.10004044896688678No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA253.7054633E-8145.027151
GGACCGG100.0070897243145.002993
TCTCCGC650.0111.540764145
AGCGAGC501.9463187E-10101.502106145
GTACTGA800.090.626876
CACGAGC259.3841384E-487.00181145
CAGGTAC850.085.295883
GCGAAAG1500.077.341362
ATGCCGG502.3048342E-672.5015145
CGAAAGC1650.070.304483
GGTACTG1150.069.349265
AGGTACT1200.066.45974
GAGCCCC554.059133E-665.91045145
GTAGAAA5300.064.304491
GCCCACC1150.063.044785145
CTCCGAC702.3326174E-762.144142145
GGTACTT350.003567870762.13387
TAAATTA606.8014633E-660.41791145
CGTCTTG400.0060492254.376125145
CCACGAC400.0060492254.376125145
Produced by FastQC (version 0.11.5)