FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_HBEpC_HIGH_1DPI_PR8_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_HBEpC_HIGH_1DPI_PR8_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1799201
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA85270.47393259563550705No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG79830.4436969521470919No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG60290.3350931885876008No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT57550.3198642063893917No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG52280.2905734267599896No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT49710.27628930842079347No Hit
GAGTTATGGGCTGGCCTCGTACAAAATTTTCAAGATCGAAAAGGGGAAGG49490.27506654342677667No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT44100.24510880107336533No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA40680.22610036343910436No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG39910.2218206859600456No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA39860.22154278482504178No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA38590.21448409599594487No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT37110.20625822239983194No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA35130.19525333745368081No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA34970.19436405382166863No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT34790.1933636097356549No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT34180.18997321588860833No Hit
CCATCACTCGGGCCATCAGTCATTATAGTAAAACATGAACCATTTACACA32950.18313684796751448No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT31920.17741208458643587No Hit
CCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGT30500.16951969235232753No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA30480.169408531898326No Hit
GTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATC28920.16073801648620695No Hit
GTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATGG28450.15812574581717107No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC26890.14945523040505201No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT26690.14834362586503674No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA26430.1468985399630169No Hit
GAGTGATGGGCTGGCCTCGTACAAAATTTTCAAGATCGAAAAGGGGAAGG25690.14278560316496045No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG25590.14222980089495282No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT25590.14222980089495282No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA25510.14178515907894673No Hit
GCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTG25040.13917288840991085No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA24970.1387838268209055No Hit
ATACTGGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGG24780.13772780250789102No Hit
GGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTGGATCAATCTGT24730.1374499013728872No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA24700.1372831606918849No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA24540.1363938770598727No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC24460.1359492352438666No Hit
CCATTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAAC23580.13105817526779942No Hit
CCATTGGATCAATCTGTCTGGTAGTCGGACTAATTAGCCTAATATTGCAA23220.12905728709577197No Hit
GCTATACCCTGACCAATCAGTCATTGCCACAACATCTTGCCTAACAGAGA23060.12816800346375973No Hit
GCACTGGAGCTAGGATGAGTCCCAATGGTTCTCATCGCTTGCACCATTTG22520.12516667120571853No Hit
CTGCAGATGTATCCTATTTGATAATCCAGGTTTTGATCGAAAGACACCCA21990.12222091917467809No Hit
CCATTATCTGGACCTGAAATTCCGATTGTTAGCCAGCCCATGCCATCATG21740.12083141349965901No Hit
GGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGGGTAAA21720.12072025304565748No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA21720.12072025304565748No Hit
GCCTTAGCTGTAGTGCTGGCTAAAACCATTCTGTTCTCATGTCTGATTAG21610.12010887054864909No Hit
CATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGGAACAG21420.11905284623563459No Hit
TCCCCGTACAATTCAAGATTTGAATCGGTTGCTTGGTCAGCAAGTGCATG21330.11855262419262773No Hit
GAATATAATCTCAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAA21200.11783008124161781No Hit
GTACGGGGACGGAGCTTCACCGACAGGGCAGCTCATTAAGGCCCTATAAG21040.11694079760960559No Hit
GTCTCTGTCCATCCATTAGGATCCCAAATCATCTCAAACCCATGTCTGGA20980.11660731624760101No Hit
GGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCTCCAACTTTTTA20920.11627383488559645No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC20550.1142173664865682No Hit
GTATCACTATTCACGCCACAAAAAGAAATGCTGCTCGCACTAGTCCAGAT20440.11360598398955982No Hit
GTCCCAATGGTTCTCATCGCTTGCACCATTTGTCTAGCCTGACTAGCAAC19910.11066023195851937No Hit
GTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGACATGGG19720.10960420764550487No Hit
TCTCAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACT19680.10938188673750181No Hit
CTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTG19330.10743657879247512No Hit
GCCCATCACTCGGGCCATCAGTCATTATAGTAAAACATGAACCATTTACA19310.10732541833847359No Hit
GCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGTACAATTCAAG19290.10721425788447206No Hit
GTCTGGAACTGTGACTTTTGGTCCTTCCTATCCAAACACCATTACCATAC19160.10649171493346213No Hit
TGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGACATG19150.10643613470646136No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT19080.10604707311745604No Hit
TAATGGAGCAGTGGCTGTATTAAAATACAACGGCATAATAACTGAAACCA19080.10604707311745604No Hit
GTTCCAGACATGGGTTTGAGATGATTTGGGATCCTAATGGATGGACAGAG19040.10582475220945298No Hit
CACCATTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCA19000.10560243130144992No Hit
ATCAAATAGGATACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCC18940.10526894993944534No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG18820.10460198721543618No Hit
GGATACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGG18740.10415734539943007No Hit
GGATCAATCTGTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGG18710.10399060471842779No Hit
AATTAGCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCC18480.10271225949741024No Hit
GAATGTGCCTGTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCC18000.10004440860137362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCCG1100.0112.04657145
AGCGAAA4950.0101.075661
GAGCGAA1750.095.2999041
GGTCGAG259.3876035E-487.00086145
CCTAGCG259.3876035E-487.00086145
GCAAAAG28500.080.397943
TTCCGCG406.6643035E-572.500725145
GCAGGGG17050.070.152249
GCACACC1800.068.47291145
CACGAGC754.8567017E-967.66734145
AGCAAAA34100.067.620042
GGGCTTA350.003566825862.143475145
CAAAAGC37550.061.2141724
AGCAGGG24900.059.09888
ATTAGTA1251.8189894E-1258.0086361
AACAGAC6000.057.994137
AAGCAGG42250.057.822557
GGCAGCC1400.056.96485145
TACATAC400.006048103354.3831023
CTTCGAC400.0060514354.37554145
Produced by FastQC (version 0.11.5)