FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_HBEpC_HIGH_12HPI_PR8_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_HBEpC_HIGH_12HPI_PR8_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1895651
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA64930.3425208543133731No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG64080.33803690658248803No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT52150.27510338137136003No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT47080.24835795196478677No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT44870.23669968786448561No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG43380.22883959125387532No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA43290.22836482031766397No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA42890.22625472726783571No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT41910.22108499929575642No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG41440.21860563996220825No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA39040.20594508166323863No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT37900.1999313164712281No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA37100.19571113037157153No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA35830.1890115849383668No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA35560.18758727212973275No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA34610.1825758011363906No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA33360.17598176035567728No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT30630.16158037529059938No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA30230.15947028224077112No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC29750.1569381705809772No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT29300.1545643158999204No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG28690.15134642399893228No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT27950.14744275185675002No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA27830.14680972394180153No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG27250.14375008901955055No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA26580.1402156831610882No Hit
GCACTGGAGCTAGGATGAGTCCCAATGGTTCTCATCGCTTGCACCATTTG26380.13916063663617406No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG25930.13678678195511726No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC25930.13678678195511726No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT25530.134676688905289No Hit
GCCTTAGCTGTAGTGCTGGCTAAAACCATTCTGTTCTCATGTCTGATTAG25470.13436017494781474No Hit
CCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGT25300.13346338540163777No Hit
CCATTATCTGGACCTGAAATTCCGATTGTTAGCCAGCCCATGCCATCATG25160.13272485283419785No Hit
GGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTGGATCAATCTGT24750.13056200745812388No Hit
CTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTG24580.12966521791194688No Hit
GTCCCAATGGTTCTCATCGCTTGCACCATTTGTCTAGCCTGACTAGCAAC23710.1250757655285704No Hit
GAATATAATCTCAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAA23260.1227019108475136No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC23200.12238539689003936No Hit
GGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGGGTAAA23190.12233264456379365No Hit
GTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATGG22960.1211193410601424No Hit
GACTTGAAGATGTCTTTGCAGGGAAGAACACTGATCTTGAGGTTCTCATG22920.12090833175515958No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT22870.12064457012393104No Hit
CCTTTCAGTCCGTATTTAAAGCGACGGTAAATGCATTTGAAAAAAAGACG22500.11869273405283991No Hit
GAAGAACACTGATCTTGAGGTTCTCATGGAATGGCTAAAGACAAGACCAA22430.11832346776911994No Hit
GTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGACATGGG22430.11832346776911994No Hit
GTCAAGTGCAAGATCCCAATGATATTTGCGGCAATAGCGAGAGGATCACT22230.1172684212442058No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG22040.1162661270455374No Hit
ATACTGGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGG21620.11405052934321772No Hit
CTCTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT21510.11347025375451494No Hit
CTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGGCCTATC21240.11204594094588086No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGCACTCCTTCCG21230.11199318861963516No Hit
CCATTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAAC21220.11194043629338946No Hit
CAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGTATGTG20950.11051612348475537No Hit
TGTCTAGCCTGACTAGCAACCTCCATGGCCTCTGCTGCTTGCTCACTCGA20480.10803676415120717No Hit
TCTCAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACT20470.10798401182496144No Hit
TGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGACATG20270.10692896530004731No Hit
GGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCTCCAACTTTTTA20230.1067179559950645No Hit
CTGCAGATGTATCCTATTTGATAATCCAGGTTTTGATCGAAAGACACCCA19980.10539914783892182No Hit
GTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATC19800.1044496059664991No Hit
ATCAAATAGGATACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCC19800.1044496059664991No Hit
GGCTAAAACCATTCTGTTCTCATGTCTGATTAGTGGATTGGTTGTTGTCA19690.10386933037779633No Hit
GTATCACTATTCACGCCACAAAAAGAAATGCTGCTCGCACTAGTCCAGAT19640.1036055687465678No Hit
GTACGGGGACGGAGCTTCACCGACAGGGCAGCTCATTAAGGCCCTATAAG19540.10307804548411073No Hit
TAATGGAGCAGTGGCTGTATTAAAATACAACGGCATAATAACTGAAACCA19500.1028670361791279No Hit
GTTCCAGACATGGGTTTGAGATGATTTGGGATCCTAATGGATGGACAGAG19490.1028142838528822No Hit
GCTAAAGACAAGACCAATCCTGTCACCTCTGACTAAGGGGATTTTAGGAT19300.10181198965421377No Hit
TACCAGGCCAAATGCCACTTCAGTGGTCACAGCCCCCATCCTGTTGTATA19260.10160098034923096No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACG100.007085452145.040424
GATCGTA100.0070932806144.98686145
CGTTACG100.0070932806144.98686145
GAGCGAA1850.0125.446991
CTTCGAC203.8679907E-4108.74015145
CCTCGCG352.764209E-7103.56205145
ACGGCCG1050.096.65791145
GTTAGCG406.1229E-790.61679145
GCAAAAG22000.086.694623
AGCGAAA4700.086.411621
TAGGCCG451.2339788E-680.548256145
GGCAGCC1450.074.99321145
GTACGAT300.001933870872.520213
CACGAGC406.6677174E-572.49343145
GCTGTCG300.001936706772.49343145
AGCAAAA27000.071.714432
GCAGGGT5150.067.561359
ACAAGGG4700.066.348283
TCCCCCC554.067102E-665.89799
AAGGGTG4750.064.123135
Produced by FastQC (version 0.11.5)