FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_DARPA_DVG_P1_PB2_PLASMID.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_DARPA_DVG_P1_PB2_PLASMID.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1882701
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT101650.5399157911957342No Hit
ATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAA93140.494714774146293No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT92140.4894032562791436No Hit
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA88080.4678384937385172No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG87860.46666995980774434No Hit
CTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATT83110.4414402499387847No Hit
GAACAGAGAAACTGACAATAACTTACTCATCGTCAATGATGTGGGAGATT76680.4072871900530142No Hit
AACTTACTCATCGTCAATGATGTGGGAGATTAATGGTCCTGAATCAGTGT65480.3477981899409412No Hit
GTATCAAATGTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTAC63840.33908730063881626No Hit
GTTTTATTATCTGTGCGGTATCAAATGTCCCAAGCACATCCCTCATTTGT62520.33207609705417906No Hit
GTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACT61550.32692392472304416No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA59860.3179474595275617No Hit
TTATTGTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTC58670.311626753265654No Hit
GACAATAACTTACTCATCGTCAATGATGTGGGAGATTAATGGTCCTGAAT55290.29367382287468907No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC54660.290327566618385No Hit
GTACTAAAGACTGAAATGGTTCAAATTCCATTTTATTGTATAGCATTGTA54450.2892121478662836No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT52600.2793858398120573No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT50030.2657352388934833No Hit
GGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTC49890.26499162639208246No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC49810.2645667049627105No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA49730.26414178353333856No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC49340.26207029156515027No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT49230.26148602459976383No Hit
GCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTG48180.25590893083925703No Hit
GTGAAACACAGGGAACAGAGAAACTGACAATAACTTACTCATCGTCAATG46490.24693246564377455No Hit
CTACAATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTA45970.24417047635285688No Hit
ATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTCAGTGG45830.24342686385145595No Hit
GTGTTGGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAA43280.22988249329022503No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT42420.2253145879244766No Hit
TTGCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATAT42220.22425228435104672No Hit
ATCCCAATCATTCCCATCACATTGTCGATAGGTTCAACTCCCCAATTTTG40580.21554139504892175No Hit
GATTAATGGTCCTGAATCAGTGTTGGTCAATACCTATCAATGGATCATCA39720.21097348968317328No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG39130.20783969414155515No Hit
GTCTGATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCAT38640.2052370503866519No Hit
GTTTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCC37330.19827896198068626No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC35760.18993987892926173No Hit
TGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA35330.1876559262463875No Hit
CAATAATTGTGGCCATGGTATTTTCACAAGAGGATTGTATGATAAAAGCA34760.18462836106211236No Hit
ATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAAGGCCA33990.18053849230440733No Hit
GTATTTTCACAAGAGGATTGTATGATAAAAGCAGTCAGAGGTGATCTGAA33690.17894503694426253No Hit
TCAATGATGTGGGAGATTAATGGTCCTGAATCAGTGTTGGTCAATACCTA33590.17841388515754758No Hit
GAATCAGTGTTGGTCAATACCTATCAATGGATCATCAGAAACTGGGAAAC33250.1766079690827168No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT32810.17427090122117106No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG31720.16848134674597826No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG31350.166516085135133No Hit
GTGTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAG30690.16301048334281437No Hit
GATAAAAGCAGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAATCAGC30300.16093899137462614No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT30280.16083276101728314No Hit
GTCGATAGGTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCTTCT30220.1605140699452542No Hit
GCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTA30210.16046095476658268No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA29980.15923930565713834No Hit
CTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTCTT29840.15849569315573742No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA29820.15838946279839444No Hit
GCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAA29620.15732715922496457No Hit
ATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTCAGTGGTCCCAG29600.15722092886762157No Hit
ATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCATTTCTC29220.15520255207810482No Hit
GTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGAT29160.15488386100607585No Hit
GAAATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAG28820.15307794493124507No Hit
CCATAAAGTTTGTCCTTGCTCATTTCTCTCAGGAATCATTTCCGTTATCC28730.1525999083232016No Hit
TGTTATAGTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCT28730.1525999083232016No Hit
CTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTC28540.15159071992844325No Hit
GTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATT27910.14824446367213912No Hit
CTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAATATCCCAATCAT27690.14707592974136627No Hit
ATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAA27190.14442017080779157No Hit
GCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATATGG26990.1433578672343617No Hit
GTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTGATGATCCATT26780.14224244848226034No Hit
CATTAATGCAGCGGATCCAGACATGATAAGATACATTGATGAGTTTGGAC26650.14155195115953093No Hit
GTGCTGGAGTACTCATCTACACCCATTTTGCTGATTCTCACTCCTCTCAT26510.14080833865813No Hit
GTCCTGAATCAGTGTTGGTCAATACCTATCAATGGATCATCAGAAACTGG26440.14043653240742954No Hit
CTTACAAACCCACTGTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTG25800.13703716097245394No Hit
CTTTAGTACCTAAGGCCATTAGAGGCCAATACAGTGGGTTTGTAAGAACT25680.13639977882839602No Hit
CATCTACACCCATTTTGCTGATTCTCACTCCTCTCATTGACATCTCGATG25370.1347532082895797No Hit
GTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGA24890.132203679713348No Hit
TCCCTCATTTGTTGGAACAGAGTTCTTACAAACCCACTGTATTGGCCTCT24880.13215056453467652No Hit
TTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAG24870.132097449356005No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA24750.13146006721194708No Hit
CTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACTGACAATAACT24610.1307164547105462No Hit
ATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCTGATCCCCTCACA24540.13034464845984572No Hit
GTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTTCTTCTTTCT24460.12991972703047378No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC24360.12938857524375885No Hit
CTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGATTATT24280.1289636538143869No Hit
GAAATATCCAATTACAGCAGACAAGAGGATAACGGAAATGATTCCTGAGA24280.1289636538143869No Hit
CAATTATTGCTTCGGCAATCGACTGTTCGTCTCTCCCACTCACTATCAGC24210.12859184756368644No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT24160.12832627167032898No Hit
GGTCAGTGAAACACAGGGAACAGAGAAACTGACAATAACTTACTCATCGT24140.12822004131298598No Hit
CCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTCAGTGGTCC23990.12742331363291357No Hit
GTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCTGATCCCC23990.12742331363291357No Hit
GATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCATTTCT23880.12683904666752713No Hit
TCCCAGAACCCTACAATGCTATACAATAAAATGGAATTTGAACCATTTCA23870.12678593148885564No Hit
GAATTAGCTCATCCTTCAGTTTTGGTGGATTCACATTTAAGAGAACAAGC23550.12508624577136784No Hit
GGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAGGA23530.12498001541402484No Hit
TTTATTATCTGTGCGGTATCAAATGTCCCAAGCACATCCCTCATTTGTTG23490.12476755469933887No Hit
ATTGAATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAG23340.12397082701926647No Hit
GCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACT23160.12301475380317958No Hit
TTCTCACTCCTCTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAAT23140.1229085234458366No Hit
GAAGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAA22830.12126195290702028No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCA22650.1203058796909334No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT22560.11982784308288996No Hit
ACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGATGTAT22470.11934980647484651No Hit
TTCCTATTCCACCATGTCACAGCCAGAGGTGATACCATCACTCGGTCTGA22440.11919046093883204No Hit
GTATTGACCAACACTGATTCAGGACCATTAATCTCCCACATCATTGACGA22220.11802192700805918No Hit
GAGTTCTTACAAACCCACTGTATTGGCCTCTAATGGCCTTAGGTACTAAA22210.11796881182938768No Hit
TAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGG22190.11786258147204469No Hit
AGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAAGA22020.1169596234346293No Hit
GCATATGGGCATATGTTGCCAAACTCTAAACCAAATACTCATTCTGATGT21980.11674716271994333No Hit
CTATCAGCTGAATCAATCTCCTGGTTGCTTTTCTGAGTATGGCTGTTGCT21760.11557862878917047No Hit
TCCTAATTCCACCAATCTGTGTGCTGTGGCACATCTCCAATAAAGATGCT21720.1153661680744845No Hit
GTAAATGAGGAGAACTGCATTCTACTTTGCTTTGGTGGAGCGGCTGCGAA21580.11462255557308355No Hit
GTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGATGT21460.11398517342902563No Hit
ACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACA21330.11329467610629622No Hit
AGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATTGTATAGCATTG21030.11170122074615141No Hit
TAATAAAACTTCTTCCCTTCGCAGCCGCTCCACCAAAGCAAAGTAGAATG21010.11159499038880842No Hit
GTTATAGTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCTG20960.11132941449545095No Hit
TTCCTGATCCCCTCACATTCACAGTAAATGAGGAGAACTGCATTCTACTT20910.11106383860209348No Hit
CTGTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAA20800.11047957163670705No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT19950.10596478144963009No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT19920.1058054359136156No Hit
GTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGA19880.10559297519892963No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC19650.10437132608948528No Hit
TCTGTGTGCTGTGGCACATCTCCAATAAAGATGCTAGTGGATCTGCTGAT19640.10431821091081378No Hit
CTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTT19600.1041057501961278No Hit
CAAGTATTCTCATTCCTGATCCCCTCACATTCACAGTAAATGAGGAGAAC19560.10389328948144183No Hit
GGATACTAACATCGGAATCGCAACTAACGATAACCAAAGAGAAGAAAGAA19560.10389328948144183No Hit
CAGTAAATGAGGAGAACTGCATTCTACTTTGCTTTGGTGGAGCGGCTGCG19270.1023529492999685No Hit
CTTCTGAAAATGTCTTAAAAGTTGATGCATAGGATTCAATCGCTGATTCG18990.10086572429716667No Hit
GGCCAATACAGTGGGTTTGTAAGAACTCTGTTCCAACAAATGAGGGATGT18960.10070637876115218No Hit
CTCTTGACTGATGATCCGCTTGTTCTCTTAAATGTGAATCCACCAAAACT18920.10049391804646621No Hit
CCCACTGTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTT18860.10017522697443726No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGACG100.0070914417144.99942145
AACCGGC256.4664982E-6115.99953145
GAATGGC458.54925E-11112.77733145
TTTCGCG404.818503E-9108.74956145
GCTTGCG301.5998601E-596.666275145
AGGACCC554.1654857E-1092.272354145
TATATAT7600.089.6706852
GTATATA7700.088.510831
CCGTTCC502.2699169E-886.99964145
GCACACC1100.085.68147145
GGGAATT353.438135E-582.85681145
ACCTACC554.3966793E-879.09058145
GCCCACC2300.078.80403145
TATAAAT1200.078.54135145
TGGTATA950.076.31548145
ATATGAG9000.075.721925
CCGGGAT300.001936039872.49971145
CTTGGCC903.6379788E-1272.49971145
CAGGGCG608.0357495E-872.499714
GTGTTTA350.00356704962.14261145
Produced by FastQC (version 0.11.5)