FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_A549_LOW_3DPI_PR8_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_A549_LOW_3DPI_PR8_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2803464
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA112440.4010752412015992No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG105040.3746793252918532No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG70420.25118924302220397No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT68140.2430564473094714No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG66200.23613643692232184No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT59910.21369990839903777No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA53000.1890518301643966No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT51670.1843076993319693No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA49550.17674562612539343No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT48910.17446273610076676No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG48600.17335696124508823No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA46350.16533117600226005No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT45090.1608367362662763No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA44440.15851817608501484No Hit
GAGTTATGGGCTGGCCTCGTACAAAATTTTCAAGATCGAAAAGGGGAAGG42770.1525612599270046No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA42730.15241857930046543No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT42470.15149115522796083No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA42460.15145548507132606No Hit
CCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGT40940.1460336212628377No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT40500.14446413437090685No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA40200.1433940296718631No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT39470.1407901082375233No Hit
CTTGTGTCCTCAATATTTTCTTCCTCCAACTTTTTATGGTTTCAGTTATT39430.14064742761098412No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA39240.13996969463492306No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT38410.13700907163423534No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT38060.13576061615201765No Hit
GTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATGG37920.13526123395913056No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA37190.13265731252479077No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA35920.1281272026321722No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC35550.1268074068366849No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG35360.12612967386062385No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA35140.12534493041465844No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA34770.12402513461917113No Hit
CCATTATCTGGACCTGAAATTCCGATTGTTAGCCAGCCCATGCCATCATG34140.12177791475117924No Hit
ATATTATTGTGTCTCCGGGTTTTAGCAAGGTCCAGTAATAGTTCATCCTC33720.12027976817251801No Hit
CCATTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAAC33300.11878162159385673No Hit
ATATAATGCAGAATTGTTAGTTCTACTGGAAAATGAAAGGACTCTGGATT32980.1176401765815434No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC32820.11706945407538674No Hit
GTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGACATGGG32800.11699811376211715No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA32250.1150362551472036No Hit
GTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATC32090.11446553264104692No Hit
ATACTGGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGG31930.11389481013489026No Hit
CTGCAGATGTATCCTATTTGATAATCCAGGTTTTGATCGAAAGACACCCA31320.11171893058016795No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG30840.11000676306169796No Hit
GTACGGGGACGGAGCTTCACCGACAGGGCAGCTCATTAAGGCCCTATAAG30600.10915067930246296No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC30200.10772387303707127No Hit
TCCCCGTACAATTCAAGATTTGAATCGGTTGCTTGGTCAGCAAGTGCATG30160.10758119241053213No Hit
CATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGGAACAG30100.10736717147072337No Hit
GTACAAGGACTTCTTTCCCTTTTTTGTTCACATAAGAATTTTTCAGCTTT30090.10733150131408857No Hit
GAGTGATGGGCTGGCCTCGTACAAAATTTTCAAGATCGAAAAGGGGAAGG29910.10668943849466232No Hit
TGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGACATG29830.10640407724158399No Hit
GGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGGGTAAA29760.10615438614514044No Hit
GTTCCAGACATGGGTTTGAGATGATTTGGGATCCTAATGGATGGACAGAG29290.10447788878330522No Hit
GGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTGGATCAATCTGT29000.10344345424089627No Hit
CAATAATATTTGAGGCAAATGGAAATCTAATAGCACCAATGTATGCTTTC28480.10158860609588709No Hit
GAATATAATCTCAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAA28460.1015172657826175No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG28070.10012612967386061No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGA100.007087077145.030531
GAGCGAA2850.099.231411
GTTAGCG750.096.66288145
ACGGCCG1550.093.544716145
CTTCGAC502.2708264E-886.99659145
TATTAGT703.092282E-1182.874581
GCAAAAG33250.077.857093
AGCGAAA5750.076.929241
CCTCGCG300.001936409472.497154145
AGCAAAA40400.064.616412
CACGAGC451.1944645E-464.44192145
AATACTA6900.064.106552
GCAGGGG18550.062.916459
AGGGTGT5450.062.534986
ACAAGGG7150.061.8650633
CAAGGGT6950.061.5586174
GCACACC2550.059.703537145
AAGGGTG5800.058.7613225
TACTATT7700.057.4461334
CAATACG400.00604698154.386451
Produced by FastQC (version 0.11.5)