FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_A549_LOW_3DPI_H3N2_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_A549_LOW_3DPI_H3N2_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2149993
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT46120.214512326319202No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC40200.18697735294952122No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA37490.1743726607481978No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT35300.16418658107258954No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA28590.13297717713499532No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC27790.12925623478774118No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA26700.12418645083960737No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA25350.11790736062861601No Hit
CTTTTAAGGCCGTGTTTGAAGGGTCGATAGACGCATTTGAAAAAAAGACG25320.11776782529059396No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC23850.11093059372751446No Hit
GTTCTTATCTCCTGTTCTACTTCAAACAGCAGTTGTAATGCTTGCATGAA23790.11065152305147041No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC21940.10204684387344516No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6250.061.4943051
CGAAAGC7750.051.4587973
GCGAAAG7900.050.4840772
GAGCGAA5100.039.8131831
GCAAAAG40400.036.255063
AGGGTGT16100.034.6787576
CAGTACG2103.8016879E-1034.518199
CACGAGC4650.032.73965145
GAGCAAA48050.031.9949551
TAGAAAC25750.031.8214552
AAGGGTG17750.031.4550955
CAAGGGT20150.030.2275434
CAAAAGC51200.029.5988584
TGATGCG7500.028.995289
GATAGCG1753.7820646E-628.995287
TAGCGCA1555.5094315E-528.0599489
ATAGCGC1606.839307E-527.1830738
ACAAGGG27350.027.0421663
AATCCGT2207.483577E-726.3673176
CGGTCTA22650.026.245409145
Produced by FastQC (version 0.11.5)