FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_A549_LOW_2DPI_PR8_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_A549_LOW_2DPI_PR8_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629429
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA59370.3643607668698667No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG53400.327722165249299No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG39110.24002273188951467No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG34570.21216021072412483No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT32770.2011133961651597No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT30680.18828681703836128No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT28550.17521475314358587No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA27710.17005957301606883No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG27620.16950723228812056No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA25680.15760122104123592No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT25270.15508500216947163No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT25070.15385757832958663No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA24500.15035942038591432No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA24110.14796594389813855No Hit
CCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGT23440.14385407403452374No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA23240.14262665019463872No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA23010.14121511277877097No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT22230.1364281598032194No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT22180.13612130384324816No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA21860.13415742569943212No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG21260.1304751541797771No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA20800.12765207934804154No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA20460.125565458820237No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA20420.12531997405226003No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT20300.12458351974832901No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA19120.11734171909300742No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT19060.11697349194104192No Hit
GAGTTATGGGCTGGCCTCGTACAAAATTTTCAAGATCGAAAAGGGGAAGG18960.11635978002109942No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT18650.11445727306927764No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA18420.11304573565340988No Hit
GTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGACATGGG18160.11145008466155937No Hit
CCATTATCTGGACCTGAAATTCCGATTGTTAGCCAGCCCATGCCATCATG18140.11132734227757085No Hit
GTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATGG17840.10948620651774334No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA17780.10911797936577783No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC17740.10887249459780082No Hit
GGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGGGTAAA17680.10850426744583531No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA17340.1064176469180308No Hit
CTGCAGATGTATCCTATTTGATAATCCAGGTTTTGATCGAAAGACACCCA17240.10580393499808828No Hit
CATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGGAACAG17140.10519022307814577No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG17120.10506748069415728No Hit
GGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCTCCAACTTTTTA17070.10476062473418603No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC17070.10476062473418603No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG16900.10371731447028376No Hit
GTACAAGGACTTCTTTCCCTTTTTTGTTCACATAAGAATTTTTCAGCTTT16750.10279674659037No Hit
ATCAAATAGGATACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCC16660.10224440586242174No Hit
CCATTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAAC16620.10199892109444475No Hit
GTTCCAGACATGGGTTTGAGATGATTTGGGATCCTAATGGATGGACAGAG16520.10138520917450224No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTTA100.00709148144.99855145
ACGGCCG650.0100.38362145
GAGCGAA1650.096.6894451
CTTCCCG554.1654857E-1092.271805145
GCAAAAG14500.084.519913
TATTAGT353.4339122E-582.876671
CGAGGCG554.3964974E-879.09012145
TCCTAGT300.001934153472.517082
CTAGTCT300.001934153472.517084
CTGAGCG406.6647735E-572.499275145
GGTTGCG300.001936038472.499275145
GAGCCCC955.456968E-1268.683525145
GCAGGGT5950.067.007929
AGCGAAA2950.066.371571
CCCACGC554.062991E-665.90843145
AATACTA1350.064.459632
GCACACC1500.062.832706145
GGGCTTA350.003567046762.14224145
GGCACCG350.003567046762.14224145
AATAGGC350.003567046762.14224145
Produced by FastQC (version 0.11.5)