FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_A549_LOW_1DPI_PR8_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_A549_LOW_1DPI_PR8_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566622
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA51590.32930726110063563No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG46350.29585949897294944No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT37270.23790039971352375No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT34100.21766578025841588No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG33700.21511251597385966No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG31360.20017591990920594No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT30210.19283528509110687No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA27820.17757953099088358No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA27510.1756007511703525No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT26980.17221767599331556No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA26790.17100487545815135No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA24080.15370650993028312No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA23290.14866381296828463No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA23280.14859998136117072No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA22770.14534456939836157No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA22670.14470625332722253No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG22500.14362111600628613No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT21800.13915290350831278No Hit
CCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGT21440.13685496565221222No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT21230.13551450190282022No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT20580.13136544744041637No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA20420.1303441417265939No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT20140.12855685672740455No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG19630.12530144476459543No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC19500.12447163387211466No Hit
GCCTTAGCTGTAGTGCTGGCTAAAACCATTCTGTTCTCATGTCTGATTAG18870.12045024262393864No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA18600.11872678923186321No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC18550.11840763119629369No Hit
GCACTGGAGCTAGGATGAGTCCCAATGGTTCTCATCGCTTGCACCATTTG18180.11604586173307921No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA17900.11425857673388985No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT17880.11413091351966205No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA17730.11317343941295346No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT17580.11221596530624489No Hit
GGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGGGTAAA17350.11074783834262508No Hit
CCATTATCTGGACCTGAAATTCCGATTGTTAGCCAGCCCATGCCATCATG17310.11049251191416946No Hit
GAGTTATGGGCTGGCCTCGTACAAAATTTTCAAGATCGAAAAGGGGAAGG17310.11049251191416946No Hit
CTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTG17210.10985419584303042No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG16980.1083860688794106No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT16880.10774775280827156No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG16800.10723709995136031No Hit
CCATTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAAC16580.1058328045948544No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC16510.10538598334505708No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA16480.10519448852371535No Hit
GTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATGG16380.10455617245257631No Hit
GTCCCAATGGTTCTCATCGCTTGCACCATTTGTCTAGCCTGACTAGCAAC16340.1043008460241207No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA16180.10327954031029822No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA15970.1019390765609062No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG15840.10110926566842544No Hit
CTGCAGATGTATCCTATTTGATAATCCAGGTTTTGATCGAAAGACACCCA15690.10015179156171686No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTT100.007094072144.98065145
GAGCGAA1200.0120.848071
ACGGCCG750.0106.319145145
CGTGGGG352.764682E-7103.55761145
GCAAAAG16850.088.215513
AGCGAAA2800.082.8672561
CCTCGCG353.4401615E-582.84609145
GGCAGCC1150.081.945595145
GCAGGGT7200.075.508359
AATACTA1950.074.368052
TAAACTA1000.072.508845
GGGCTTA300.001936972772.490326145
CGTCTTG300.001936972772.490326145
CTATTCG1000.072.488029
CGAGGCG651.3998579E-766.91415145
ACAAGGG4450.065.176493
GCAGGGG6450.064.059189
AGGGTGT3400.063.978396
AGCAAAA23350.063.9692572
TACTATT2300.063.0511674
Produced by FastQC (version 0.11.5)