FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_A549_LOW_1DPI_H3N2_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_A549_LOW_1DPI_H3N2_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2080825
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT59870.28772241779102037No Hit
CTTTTAAGGCCGTGTTTGAAGGGTCGATAGACGCATTTGAAAAAAAGACG56340.2707579926231182No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC51580.2478824504703663No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT50750.24389364795213436No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA49580.23827087813727726No Hit
CTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC46900.225391371210938No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG45610.21919190705609556No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT45330.21784628692946306No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA45060.21654872466449604No Hit
ACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCTGCTGT43240.20780219384138504No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC42330.20342892842982954No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA39060.18771400766522894No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC37420.17983251835209593No Hit
CCATTATATGATGCGATCAAGTGCATAAAAACATTCTTTGGATGGAAAGA37400.17973640262876503No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA33500.1609938365792414No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA32750.15738949695433302No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA32310.15527495104105343No Hit
GTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCCTGCACCATTCG32290.15517883531772253No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG32280.15513077745605708No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC30890.14845073468456021No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA30870.1483546189612293No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC30250.1453750315379717No Hit
GTTCTTATCTCCTGTTCTACTTCAAACAGCAGTTGTAATGCTTGCATGAA30010.14422164285800104No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT28480.13686879002318791No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG28060.1348503598332392No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC28050.13480230197157378No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT27900.1340814340465921No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC26780.12869895354006225No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT26670.12817031706174234No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG26640.128026143476746No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG26480.1272572176900989No Hit
CTCCCAGCACAGGTCTCATAGGCAAATGGTGGCAACAACCAACCCATTAA25430.12221114221522714No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA24410.11730924032535173No Hit
GGGTAAGGGATATCCCCAGCAGAATAAGCATCTATTGGACAATAGTAAAA24370.11711700887868995No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG24070.11567527302872659No Hit
CCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTGTCATTGGGAATG23830.1145218843487559No Hit
CTCCAACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCT23640.11360878497711244No Hit
CTCCTGTTCTACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT23590.11336849566878522No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT23590.11336849566878522No Hit
CATTAATACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATTTTC23410.11250345415880719No Hit
ATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTCAAC23300.11197481768048731No Hit
CTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAG23030.11067725541552029No Hit
ACCTTATATAGTCAAACCACACGAAAAGGGAATAAATTCAAATTACCTAC22440.10784184157725903No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTAT22250.10692874220561556No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG22210.10673651075895378No Hit
ATACTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTT22190.10664039503562289No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG21890.10519865918565954No Hit
ACATACAATTGATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAGAA21890.10519865918565954No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG21740.10447779126067785No Hit
TCTCTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCA21670.10414138622901972No Hit
TCCTAGCTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA21630.10394915478235794No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG21510.10337246044237261No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA21450.10308411327237994No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT21250.10212295603907104No Hit
ACTAGAGACCATAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTG21160.10169043528408203No Hit
CTTATCTCCTGTTCTACTTCAAACAGCAGTTGTAATGCTTGCATGAATGT20860.10024869943411868No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC100.0070871613145.029076
CACGAGC750.0106.331635145
AGGCTCG850.0102.35131145
GTCCTAA259.3887775E-486.99862145
TCTCCGC750.086.99861145
GCAGGGG3650.077.457089
TAAACTA1200.072.5145345
TATAGTG300.001934498472.5145345
CCTAGCG300.001936159272.49885145
GCCGGGG300.001936159272.49885145
CCCACGC406.665403E-572.49885145
GTCAAGG754.8585207E-967.66559145
GGTAGGA651.3996578E-766.917187
TCACGCA350.003567269562.14187145
GCAGGGT9250.061.9119839
CAGTACG2500.060.8931739
AGGGTGT12650.057.323746
GAGCCCC1600.054.374138145
TAGGACC8350.053.826319
TTTAAAC1650.052.7378433
Produced by FastQC (version 0.11.5)