FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_A549_LOW_12HPI_H3N2_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_A549_LOW_12HPI_H3N2_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478554
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGGGTCGATAGACGCATTTGAAAAAAAGACG55490.375299109805932No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA49320.33356914931750886No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC45690.30901813528623234No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG43690.2954914057924161No Hit
ACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCTGCTGT41960.28379078478026504No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC41300.27932696404730567No Hit
CTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC37920.25646679120275623No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT36180.24469853654313606No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG34930.23624433060950092No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA30640.20722949584526504No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA29480.1993839927388516No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA28310.1914708559849691No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC26820.18139344251207598No Hit
GTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCCTGCACCATTCG26710.1806494723899161No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT26430.17875573026078181No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG26400.17855282931837457No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG25940.17544168153479683No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG25610.17320977116831715No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA24490.16563480265178004No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA24010.16238838757326415No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG23890.16157678380363516No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTAT23400.15826273507765018No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC23390.15819510143018112No Hit
CTCCAACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCT22770.15400181528709805No Hit
CTCCCAGCACAGGTCTCATAGGCAAATGGTGGCAACAACCAACCCATTAA21720.14690028230284455No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT21620.14622394582815373No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA20600.13932531378630744No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA20410.13804027448439488No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG19680.13310301821915196No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC19610.1326295826868684No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT19400.13120927609001767No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT19030.1287068311336617No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT18950.12816576195390902No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG18900.1278275937165636No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC18810.12721889088934188No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGGTCAAGTGAGCAGGCA18550.12546041605514577No Hit
TCCTAGCTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA18500.12512224781780037No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA18130.12261980286144436No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT17960.12147003085446997No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC17870.12086132802724824No Hit
GCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTGGGGT17830.12059079343737192No Hit
GTGCTGGGAGTCAGCAATCTGCTCACATGTTGCACATACCAGGCCAAATG17070.11545063622972174No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT17060.11538300258225266No Hit
TACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATTGTATA16930.1145037651651546No Hit
CATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCA16750.11328635951071114No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC16490.11152788467651503No Hit
ATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTCAAC16380.11078391455435514No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT16280.11010757807966433No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTGGGG15520.10496742087201415No Hit
GATATTGAGAGGGTCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCGT15270.10327657968528711No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG15130.10232970862071997No Hit
CTCATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCT15120.10226207497325088No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC15030.10165337214602917No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT14860.10050360013905478No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGCG100.007093181144.98647145
CCGTATA100.007093181144.98647145
CGGCTCG100.007093181144.98647145
AGGCTCG1100.0125.21558145
CCACGAC406.122318E-790.61655145
GTCCACG300.001935603472.503053
CGAGTCG300.001936642672.49324145
CGGGAGC406.667321E-572.49323145
TCTCCGC1100.072.49323145
AGGGTGT6000.070.086286
GCAGGGG3100.063.1350029
GCAGGGT10800.062.4205139
TTTAGCG350.003566247462.1454662
TAGCGCA350.003566247462.1454664
TCCACGC350.003566247462.1454664
CCACGCT350.003566247462.1454665
CAGTACG1300.061.3362859
GAGTCGC606.8109657E-660.411034145
AGCGAAA5150.059.1306951
CGAAAGC5400.056.391263
Produced by FastQC (version 0.11.5)