FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_A549_HIGH_1DPI_H1N1_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_A549_HIGH_1DPI_H1N1_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1937619
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA33750.050.490011
GCGAAAG9400.045.508312
CGAAAGC10350.042.0317463
GCAAAAG42650.038.9298743
AGCGAAA11300.037.860381
CAAAAGC56900.030.3271244
CGCGGCG1255.156736E-428.998909145
GACCGAG8500.028.9944217
TGACCGA7950.028.2722996
AGCAAAA59100.028.094062
GAGCGAA7800.027.8893261
CTACGCG1306.482669E-427.8864485
ATGACCG8900.026.0691285
ACCGAGC9600.025.6721448
TGACCGG3709.094947E-1225.4680739
AAGCAGG76200.025.0181527
ACGCGGG1801.5229896E-424.162027
AAAGCAG79150.024.0919156
AAAAGCA80550.021.6929235
TAGAAAC22150.020.9494572
Produced by FastQC (version 0.11.5)