FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_A549_HIGH_12HPI_PR8_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_A549_HIGH_12HPI_PR8_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640480
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA65310.39811518579927824No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG59280.3613576514190968No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT47130.2872939627426119No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG42660.26004584024188043No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT41690.2541329367014532No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA34930.21292548522383695No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA34010.2073173705256998No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG33710.20548863747195945No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG32910.20061201599531844No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT32810.2000024383107383No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA30160.18384862966936505No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA29760.18141031893104456No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA29650.18073978347800643No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT29190.17793572612893788No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA28440.17336389349458695No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT28090.17123037159855653No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT27870.16988930069248023No Hit
GAGTTATGGGCTGGCCTCGTACAAAATTTTCAAGATCGAAAAGGGGAAGG27330.1665975811957476No Hit
CCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGT25570.15586901394713742No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA25490.15538135179947332No Hit
GCACTGGAGCTAGGATGAGTCCCAATGGTTCTCATCGCTTGCACCATTTG24520.14946844825904612No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC24320.1482492928898859No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT23720.14459182678240515No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT23640.14410416463474107No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA23610.143921291329367No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA23200.1414220228225885No Hit
GTCCCAATGGTTCTCATCGCTTGCACCATTTGTCTAGCCTGACTAGCAAC23080.14069052960109235No Hit
GCCTTAGCTGTAGTGCTGGCTAAAACCATTCTGTTCTCATGTCTGATTAG21880.1333755973861309No Hit
CTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTG21090.1285599336779479No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGCACTCCTTCCG21070.1284380181410319No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT20570.12539012971813127No Hit
GAAGAACACTGATCTTGAGGTTCTCATGGAATGGCTAAAGACAAGACCAA20500.1249634253389252No Hit
GTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATGG20410.12441480542280307No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG20280.12362235443284893No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA19720.12020871939920022No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG19210.1170998732078416No Hit
GACTTGAAGATGTCTTTGCAGGGAAGAACACTGATCTTGAGGTTCTCATG19020.11594167560713937No Hit
GCTATACCCTGACCAATCAGTCATTGCCACAACATCTTGCCTAACAGAGA18420.11228420949965863No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT18350.11185750512045256No Hit
CCATCACTCGGGCCATCAGTCATTATAGTAAAACATGAACCATTTACACA18180.11082122305666633No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA17800.10850482785526189No Hit
CTCTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT17710.10795620793913976No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT17700.10789525017068176No Hit
GGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCTCCAACTTTTTA17580.1071637569491856No Hit
CCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGC17580.1071637569491856No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG17480.10655417926460549No Hit
CAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGTATGTG17370.10588364381156734No Hit
TGTCTAGCCTGACTAGCAACCTCCATGGCCTCTGCTGCTTGCTCACTCGA17350.10576172827465131No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA17320.10557885496927728No Hit
GCACATACCAGGCCAAATGCCACTTCAGTGGTCACAGCCCCCATCCTGTT17310.10551789720081926No Hit
CATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGGAACAG17140.10448161513703307No Hit
GGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGGGTAAA16900.10301862869404076No Hit
GGCTAAAACCATTCTGTTCTCATGTCTGATTAGTGGATTGGTTGTTGTCA16780.10228713547254462No Hit
CCATTATCTGGACCTGAAATTCCGATTGTTAGCCAGCCCATGCCATCATG16760.10216521993562859No Hit
ATACTGGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGG16630.10137276894567444No Hit
GTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATC16580.10106798010338439No Hit
TCCCCGTACAATTCAAGATTTGAATCGGTTGCTTGGTCAGCAAGTGCATG16430.10015361357651419No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC16420.10009265580805618No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCGC100.007088323145.020191
TGCCGAG100.007088969145.015764
CCTCGCG202.1308351E-6145.0025145
GAACGCG100.0070909075145.0025145
GAGCGAA1650.0109.863771
CTGTCTA501.9463187E-10101.50175145
ACGGCCG950.099.212234145
GCAAAAG20850.088.330953
AGCGAAA3650.083.436271
GGTGCCC353.4376408E-582.85857145
CTACTAA451.2324435E-680.5643166
CCGGCGC300.001935831972.50124145
GCTCACG300.001935831972.50124145
GCAGGGG10950.072.161399
AATACTA1750.070.436232
TCTCCGC1200.066.45947145
ATTAGTA554.0594077E-665.918271
TCTACTA554.0601426E-665.916255
TACTAAT554.0652867E-665.902197
TATTAGT451.1932632E-464.4534151
Produced by FastQC (version 0.11.5)