FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_A549_HIGH_12HPI_H1N1_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_A549_HIGH_12HPI_H1N1_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038077
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT58570.2873787398611534No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA52260.2564181824337353No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC49770.2442007833855149No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT42470.20838270585458743No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG40050.19650876782378685No Hit
CTATTGGGACTCATCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTT39750.1950367920348446No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC37610.18453669807372342No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG35500.17418380169149644No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT33890.16628419829083985No Hit
TCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG33850.1660879348523142No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA33780.16574447383489438No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC32530.1596112413809684No Hit
GGGCTAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC32410.15902245106539156No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC32120.15759954113608074No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG31470.15441026026003923No Hit
CCTTAGCCCCATGGAACGTTATTTCTCTTTTAAGCTTCTTGTATAGTTTA30830.15127004524362916No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTTAAAAGAGAAATAACGTTCC29660.1455293396667545No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACTTGGAGAAAAGAATAGAGA29510.1447933517722834No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT29410.1443026931759693No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC28010.13743347282757226No Hit
GGATTGGGTGATGCCCCATTCCTTGACCGGCTACGCCGAGATCAAAAGTC27610.13547083844231594No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC27220.13355726991669106No Hit
ATGTAGGACCATGAACTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC26420.12963200114617848No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA26250.12879788153244454No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC25660.12590299581419154No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC25620.12570673237566587No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT25540.12531420549861463No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT25340.12433288830598647No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA25260.12394036142893523No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC25130.12330250525372692No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT25030.12281184665741285No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG24500.12021135609694826No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAT23870.11712020694016959No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC23820.11687487764201256No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC23000.11285147715223713No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT22850.11211548925776603No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC22840.11206642339813462No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG22830.11201735753850321No Hit
GATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGC22800.111870159959609No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATCGTG22490.11034911831103536No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGCAG22440.11010378901287832No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA22240.10912247182025016No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA22100.10843554978541046No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC21990.10789582532946498No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC21910.10750329845241371No Hit
ATACAAGAAGCTTAAAAGAGAAATAACGTTCCATGGGGCTAAGGAGGTGT21830.10711077157536245No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC21770.10681637641757402No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT21600.10598225680384007No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT21330.10465747859379208No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA21200.10401962241858378No Hit
TTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACC21180.10392149069932098No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC20710.10161539529664483No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC20690.101517263577382No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGATAAAGC20610.10112473670033076No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCGC202.1312935E-6144.99796145
GAGCGAA2600.0119.92571
GCTCGGT203.863837E-4108.769821
GGATTAA650.0100.3832145
AGGCTCG1300.0100.3832145
AGCGAGC2800.093.212975145
CTGCACG502.2700988E-886.99878145
GCCCACC1300.083.65267145
TAGGCCG950.076.31471145
AGTAGAA8700.067.512311
GCAGGTA13950.065.996189
GCGAAAG8400.065.60722
CGAAAGC8500.064.835353
TAGTATA808.092684E-963.436604145
GTAGAAA10300.063.3610651
CTTAATC350.003564460262.1541863
GTGTTTT8150.061.3734869
CTCCGAC851.3063982E-859.705044145
GTACTGA2750.058.0105746
CCCACGC753.7635255E-757.999187145
Produced by FastQC (version 0.11.5)