FastQCFastQC Report
Sat 24 Aug 2019
HNH27AFXY_n01_A549_HIGH_12HPI_H1N1_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH27AFXY_n01_A549_HIGH_12HPI_H1N1_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684863
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT46790.2777080391699503No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA42140.2501093560722741No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC40150.23829830674660193No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT32920.1953868059302151No Hit
CTATTGGGACTCATCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTT32390.19224114957714664No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC31790.18868002917744647No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG31510.18701817299091975No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA28980.17200211530551743No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG28830.1711118352055924No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT27450.16292125828628204No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACTTGGAGAAAAGAATAGAGA27110.1609032900597853No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC27040.1604878260131536No Hit
TCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG26990.16019106597984525No Hit
GGGCTAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC26480.1571641136401001No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG26390.1566299455801451No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC26130.1550867934069417No Hit
CCTTAGCCCCATGGAACGTTATTTCTCTTTTAAGCTTCTTGTATAGTTTA25400.15075409692063985No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT24810.14725232852760137No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTTAAAAGAGAAATAACGTTCC24510.14547176832775127No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC23360.1386462875616593No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC21950.13027765462236396No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC21940.13021830261570227No Hit
ATGTAGGACCATGAACTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC21640.1284377424158522No Hit
GGATTGGGTGATGCCCCATTCCTTGACCGGCTACGCCGAGATCAAAAGTC21060.12499532602947538No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT20660.12262124576300863No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG20490.12161226164976026No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA20360.12084068556315855No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC20300.12048457352318853No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAT19880.11799178924339843No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG19650.11662669309018003No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT19520.11585511700357833No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT19510.11579576499691667No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA19510.11579576499691667No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA19280.11443066884369826No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC19200.11395585279040492No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC18910.11223464459721649No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC18890.11211594058389317No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT18640.11063214041735145No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC18480.10968250831076473No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATCGTG17970.1066555559710196No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC17940.10647749995103459No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC17890.10618073991772625No Hit
GATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGC17800.10564657185777122No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGCAG17540.10410341968456784No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT17270.10250091550470276No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT17230.10226350747805607No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA17160.10184804343142438No Hit
GTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACT17140.10172933941810106No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGG17020.10101711533816102No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC16940.10054229928486767No Hit
TTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACC16930.100482947278206No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA16920.10042359527154432No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC16870.10012683523823598No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1900.0122.119141
AGGCTCG700.0113.92461145
CGAAAGC6800.081.038623
GCGAAAG6900.079.864142
TCTCCGC950.076.31313145
CAGGTAC2350.074.0509643
TAAATTA1900.068.68182145
GCAGGTA10900.068.498669
GTAGAAA9100.067.727481
GCCCACC1100.065.9068145
GTACTGA2650.065.667836
AGTAGAA6750.065.525961
CTGCACG451.1943006E-464.4422145
GGTACTG2750.063.279925
AGCGAGC1950.063.202923145
TCACGCG350.003567418562.140694145
CACGAGC1051.6370905E-1162.140694145
ACTAGGT350.003567418562.140694145
AGGGCGC350.003567418562.140694145
AGGTACT3000.060.423534
Produced by FastQC (version 0.11.5)