FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34315222
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11053813.2212555699042253No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7825922.280597223004998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC75950.029.8362143
GGATAGC88950.028.9054055
TATACTG145400.025.5423855
ACTGATC138350.025.384268
GCAGGTA97400.023.3768652
TGATCCG31550.023.313769
ACGCGCC31850.021.1959658
TGGATAG123800.020.8884374
CAGGTAC115100.020.781523
GACAGTA104550.020.7361077
TTGGACA113500.020.641954
GTATGGA158800.020.3821751
CCTTATA135500.020.1973292
TATAGCG14750.019.6100832
GACGCGC28050.018.869927
ATACTGG156750.018.8312386
GTATAGG60800.018.0706041
TATGGAT156300.017.9840322
CGCGCCA35800.017.5539199
GGACAGT160000.017.4010246
Produced by FastQC (version 0.11.5)