FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_pDZ_PB2_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_pDZ_PB2_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530420
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA46680.30501430979731053No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC40300.2633264071300689No Hit
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT39120.2556161053828361No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT38900.2541785914977588No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC36020.2353602279112923No Hit
ATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAA35430.23150507703767592No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG34490.225362972255982No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA33500.21889415977313417No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT28410.18563531579566395No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT27630.1805386756576626No Hit
GTGCTGGAGTACTCATCTACACCCATTTTGCTGATTCTCACTCCTCTCAT27550.1800159433358163No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT27230.17792501404843114No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG27100.17707557402543092No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT26970.1762261340024307No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA26810.1751806693587381No Hit
GTGTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAG26360.17224030004835275No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT25340.16557546294481254No Hit
ATCCCAATCATTCCCATCACATTGTCGATAGGTTCAACTCCCCAATTTTG24730.16158962899073456No Hit
GTATCAAATGTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTAC24420.1595640412435802No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA24290.15871460122057998No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT22920.14976281020896226No Hit
GTCGATAGGTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCTTCT22450.14669175781811528No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG22300.14571163471465348No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA22020.14388207158819147No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC21860.1428366069444989No Hit
GCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTA21790.1423792161628834No Hit
GCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTG21640.1413990930594216No Hit
CTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATT21320.13930816377203645No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT21180.13839338220880543No Hit
GTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGAT21040.1374786006455744No Hit
GTTTTATTATCTGTGCGGTATCAAATGTCCCAAGCACATCCCTCATTTGT20980.13708655140418968No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT20880.13643313600188184No Hit
GTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACT20830.1361064283007279No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC20830.1361064283007279No Hit
GAACAGAGAAACTGACAATAACTTACTCATCGTCAATGATGTGGGAGATT20600.13460357287541982No Hit
CTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTC20510.13401549901334273No Hit
CTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAATATCCCAATCAT20400.13329674207080408No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC20290.13257798512826544No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT20160.13172854510526522No Hit
CTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGATTATT20150.13166320356503444No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA19690.12865749271441826No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG19590.12800407731211041No Hit
GATAAAAGCAGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAATCAGC19440.1270239542086486No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT19420.12689327112818705No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT19240.12571712340403288No Hit
GGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAGGA18890.12343016949595537No Hit
GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTT18800.12284209563387827No Hit
CTACAATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTA18740.12245004639249357No Hit
GGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTC18730.12238470485226277No Hit
GTATTTTCACAAGAGGATTGTATGATAAAAGCAGTCAGAGGTGATCTGAA18680.12205799715110885No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC18680.12205799715110885No Hit
ATTCTAGACGGGCCGGCCCCTGCGTGTGGCACGGGCGGCCGGGAGGGCGT18590.12146992328903178No Hit
CTCTTGACTGATGATCCGCTTGTTCTCTTAAATGTGAATCCACCAAAACT18460.12062048326603154No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC18410.12029377556487762No Hit
GTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTGATGATCCATT18310.11964036016256976No Hit
CTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTCTT18310.11964036016256976No Hit
TTCCTGATCCCCTCACATTCACAGTAAATGAGGAGAACTGCATTCTACTT18300.11957501862233895No Hit
GTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTTCTTCTTTCT18290.11950967708210818No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC18100.11826818781772325No Hit
CTATCAGCTGAATCAATCTCCTGGTTGCTTTTCTGAGTATGGCTGTTGCT18020.11774545549587695No Hit
TTATTGTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTC17760.1160465754498765No Hit
CAATTATTGCTTCGGCAATCGACTGTTCGTCTCTCCCACTCACTATCAGC17760.1160465754498765No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG17520.11447837848433765No Hit
TTCCTATTCCACCATGTCACAGCCAGAGGTGATACCATCACTCGGTCTGA17510.11441303694410684No Hit
GGATACTAACATCGGAATCGCAACTAACGATAACCAAAGAGAAGAAAGAA17490.11428235386364528No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG17390.11362893846133741No Hit
CAATAATTGTGGCCATGGTATTTTCACAAGAGGATTGTATGATAAAAGCA17330.11323688921995269No Hit
CTCCAGCACGGAGAGGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCC17150.11206074149579853No Hit
TTCTCACTCCTCTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAAT17110.11179937533487538No Hit
CTCCTGGAGTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAAC17040.11134198455325989No Hit
CATCTACACCCATTTTGCTGATTCTCACTCCTCTCATTGACATCTCGATG16930.11062322761072124No Hit
TGTTATAGTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCT16770.10957776296702866No Hit
GTCCATTTTAGAAACCAAGTCAAAATACGTCGGAGAGTTGACATAAATCC16710.10918571372564394No Hit
GTATTGACCAACACTGATTCAGGACCATTAATCTCCCACATCATTGACGA16640.10872832294402844No Hit
GTTTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCC16580.1083362737026437No Hit
GGCTAAAGCATGGAACCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAA16560.10820559062218214No Hit
GTGTTGGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAA16300.10650671057618172No Hit
TCTCAGTGCCAAGGAGGCACAGGATGTAATCATGGAAGTTGTTTTCCCTA16260.10624534441525854No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT16240.10611466133479698No Hit
CCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAAATACGTCGGAGAGTT16130.10539590439225834No Hit
AACTTACTCATCGTCAATGATGTGGGAGATTAATGGTCCTGAATCAGTGT15980.10441578128879654No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT15640.1021941689209498No Hit
CTTTAGTACCTAAGGCCATTAGAGGCCAATACAGTGGGTTTGTAAGAACT15550.10160609505887273No Hit
TCCCAGAACCCTACAATGCTATACAATAAAATGGAATTTGAACCATTTCA15530.10147541197841116No Hit
ATTGAATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAG15520.10141007043818037No Hit
GAAGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAA15420.1007566550358725No Hit
CTTCTGAAAATGTCTTAAAAGTTGATGCATAGGATTCAATCGCTGATTCG15400.10062597195541093No Hit
CTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTT15380.10049528887494937No Hit
TCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCCAGCACTAAGCATC15340.10023392271402622No Hit
AGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAAGA15320.10010323963356464No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA100.007069035145.15212
GCGTTAA203.8684296E-4108.73603145
CCGACGC700.0103.55812145
CCGTTCC700.093.20231145
CCAAACC750.086.98882145
CTTGGCC850.085.283165145
TATATAT5350.075.967452
GTATAGA406.6094006E-572.621181
TCTGACC801.0004442E-1072.490685145
TTTCGCG300.00193692672.490685145
TGGTATA300.00193692672.490685145
ATATGAG6050.068.30375
GTATATA6050.067.2196041
GTATTGC902.8194336E-1064.436165145
TATAGAA350.00355208162.2080422
CAAGACC350.003568680762.134872145
TAGGGGC2050.060.120125
TTAGGGG2100.058.6925284
TACACAA753.765872E-757.99255145
CCGCGGA651.0871476E-555.827732
Produced by FastQC (version 0.11.5)