FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_pDZ_PB2_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_pDZ_PB2_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1882966
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA60260.3200270211995331No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC58620.3113173578280224No Hit
ATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAA57230.3039353870436322No Hit
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT53480.2840199982368243No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT52680.2797713819580386No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG50900.27031821073774037No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC49640.2636266400986529No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA45640.2423835587047244No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT44650.23712589605972703No Hit
GTGTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAG44230.23489537251336454No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT37860.20106576539353338No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT37650.19995050362035216No Hit
GGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAGGA37390.1985697033297468No Hit
GTATCAAATGTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTAC36800.19543634882414232No Hit
GTGCTGGAGTACTCATCTACACCCATTTTGCTGATTCTCACTCCTCTCAT35870.19049733240005395No Hit
GCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTG35420.18810748574323702No Hit
GTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGAT35340.18768262411535844No Hit
GTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACT34640.18396508487142094No Hit
CTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATT33890.17998200711005935No Hit
GCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTA32740.17387462120930489No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA32310.17159098995945757No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT31810.16893560478521652No Hit
GAACAGAGAAACTGACAATAACTTACTCATCGTCAATGATGTGGGAGATT30850.16383726525067366No Hit
CTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGATTATT30650.16277511118097723No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT30330.16107566466946296No Hit
GTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTGATGATCCATT30170.16022594141370583No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT29840.15847338719870674No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT28990.15395923240249693No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC28910.15353437077461835No Hit
TTCCTATTCCACCATGTCACAGCCAGAGGTGATACCATCACTCGGTCTGA28450.15109141641431656No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC28280.1501885854550746No Hit
TTCCTGATCCCCTCACATTCACAGTAAATGAGGAGAACTGCATTCTACTT28190.1497106161237112No Hit
CTCCAGCACGGAGAGGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCC28170.14960440071674158No Hit
TTATTGTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTC27750.1473738771703791No Hit
GGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTC27220.14455916888568354No Hit
CTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACTGACAATAACT26830.14248796844977552No Hit
TTGCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATAT26700.14179756830447282No Hit
ATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCATTTCTC26610.14131959897310944No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT26580.14116027586265498No Hit
GTTTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCC26570.14110716815917015No Hit
ATCCCAATCATTCCCATCACATTGTCGATAGGTTCAACTCCCCAATTTTG26360.1399919063859889No Hit
GTTTTATTATCTGTGCGGTATCAAATGTCCCAAGCACATCCCTCATTTGT26240.13935461394417106No Hit
GTCTGATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCAT26080.13850489068841393No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC26050.13834556757795946No Hit
CTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTC26010.13813313676402017No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG25510.1354777515897791No Hit
CTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAATATCCCAATCAT25410.1349466745549309No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA25090.1332472280434166No Hit
CTCTTGACTGATGATCCGCTTGTTCTCTTAAATGTGAATCCACCAAAACT24710.1312291353109934No Hit
GATAAAAGCAGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAATCAGC24640.13085738138659964No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT24380.12947658109599428No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC24290.1289986117646309No Hit
GATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCATTTCT24180.12841442702629788No Hit
GTGTTGGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAA23960.1272460575496318No Hit
AACTTACTCATCGTCAATGATGTGGGAGATTAATGGTCCTGAATCAGTGT23390.12421891845099699No Hit
TCTGTGTGCTGTGGCACATCTCCAATAAAGATGCTAGTGGATCTGCTGAT23000.12214771801508896No Hit
CTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTCTT22860.12140421016630146No Hit
GGCCAATACAGTGGGTTTGTAAGAACTCTGTTCCAACAAATGAGGGATGT22830.121244887055847No Hit
GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTT22790.1210324562419077No Hit
TCCTAATTCCACCAATCTGTGTGCTGTGGCACATCTCCAATAAAGATGCT22720.12066070231751397No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT22060.11715559388751576No Hit
GTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGATGT21880.11619965522478898No Hit
GTATTGACCAACACTGATTCAGGACCATTAATCTCCCACATCATTGACGA21850.11604033211433451No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG21810.11582790130039522No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA21710.11529682426554702No Hit
CTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTT21580.11460642412024434No Hit
CTTTAGTACCTAAGGCCATTAGAGGCCAATACAGTGGGTTTGTAAGAACT21570.1145533164167595No Hit
CTATCAGCTGAATCAATCTCCTGGTTGCTTTTCTGAGTATGGCTGTTGCT21420.1137567008644872No Hit
GTCGATAGGTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCTTCT21190.11253522368433631No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC21130.11221657746342739No Hit
GTGAAACACAGGGAACAGAGAAACTGACAATAACTTACTCATCGTCAATG21070.11189793124251844No Hit
TTAGTACCTAAGGCCATTAGAGGCCAATACAGTGGGTTTGTAAGAACTCT21050.11179171583554882No Hit
CTACAATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTA20920.11110131569024614No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT20880.11088888487630684No Hit
GTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTTCTTCTTTCT20710.1099860539170649No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG20670.10977362310312559No Hit
TTATTGGAGATGTGCCACAGCACACAGATTGGTGGAATTAGGATGGTAGA20630.10956119228918632No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT20280.10770242266721756No Hit
ATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTTTGGTG20060.1065340531905515No Hit
GTATTTTCACAAGAGGATTGTATGATAAAAGCAGTCAGAGGTGATCTGAA19940.10589676074873365No Hit
CAATTATTGCTTCGGCAATCGACTGTTCGTCTCTCCCACTCACTATCAGC19870.10552500682433989No Hit
TCTCCCAACCATTGTGAACTCTTCATATCCCTCATGCACTCTTATCTTCA19840.10536568371388544No Hit
ATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTCAGTGG19830.1053125760104006No Hit
GTATCAGCAGATCCACTAGCATCTTTATTGGAGATGTGCCACAGCACACA19720.10472839127206757No Hit
ATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAA19470.10340069868494704No Hit
GGCCATTAGAGGCCAATACAGTGGGTTTGTAAGAACTCTGTTCCAACAAA19440.10324137557449259No Hit
TTCTCACTCCTCTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAAT19420.10313516016752294No Hit
GGATACTAACATCGGAATCGCAACTAACGATAACCAAAGAGAAGAAAGAA19380.10292272935358364No Hit
TCTCAGTGCCAAGGAGGCACAGGATGTAATCATGGAAGTTGTTTTCCCTA19140.10164814446994794No Hit
CTCCTGGAGTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAAC19030.1010639597316149No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAGG100.007091356145.05
GGATTCG100.007094735144.9769145
CCGTTCC800.0108.73267145
AGGACCC301.6010943E-596.65127145
GTGTTTA700.093.19943145
CCGACGC1050.082.84394145
AGGTGCG353.440785E-582.843949
GCCGACC851.8189894E-1276.75247145
TATATAT5150.076.090142
TCTGACC1150.075.64012145
GCACACC1350.075.17321145
TTTCGCG300.001937232872.48845145
GTATATA5450.071.955251
ATATGAG5850.066.923085
TCACCGC1300.066.912415145
GAGATGG1100.066.0195541
GATGGGC606.7406563E-660.5179251
AAGTATA2300.059.8865366
CAGGGCG851.3058525E-859.707474
ACTCCGC1102.5465852E-1159.3118827
Produced by FastQC (version 0.11.5)