HNG7HBGXG_n02_pDZPB2_nonamp_replicate2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HNG7HBGXG_n02_pDZPB2_nonamp_replicate2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49816
Sequences flagged as poor quality	0
Sequence length	151
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC	100	0.2007387184840212	No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT	93	0.18668700819013972	No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT	83	0.1666131363417376	No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT	82	0.16460574915689738	No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG	80	0.16059097478721696	No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG	79	0.15858358760237676	No Hit
CTATTGCAATGAAAATAAATTTCCTTTATTAGCCAGAAGTCAGATGCTCA	79	0.15858358760237676	No Hit
GTACCCGGGCATGCTCGAGCTAGCAGATCTTTTTCCCTCTGCCAAAAATT	74	0.14854665167817568	No Hit
CCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCC	73	0.14653926449333549	No Hit
TTGCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATAT	72	0.14453187730849526	No Hit
GCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA	69	0.13850971575397464	No Hit
GCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTT	68	0.1365023285691344	No Hit
ATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAA	65	0.1304801670146138	No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG	65	0.1304801670146138	No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT	65	0.1304801670146138	No Hit
GTCGATAGGTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCTTCT	63	0.12646539264493337	No Hit
GTGTGTGGCTGCGATGGTGGCGTTTTTGGGGACAGGTGTCCGTGTCGCGC	63	0.12646539264493337	No Hit
GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTT	62	0.12445800546009313	No Hit
GTTCTGAGGGGATTCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCC	61	0.12245061827525293	No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT	61	0.12245061827525293	No Hit
ACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACA	61	0.12245061827525293	No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC	61	0.12245061827525293	No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG	60	0.12044323109041272	No Hit
TTTCAGAAGGATGCGAAAGTGCTTTTTCAAAATTGGGGAGTTGAACCTAT	58	0.1164284567207323	No Hit
ATTGAATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAG	58	0.1164284567207323	No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG	58	0.1164284567207323	No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG	56	0.11241368235105187	No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA	56	0.11241368235105187	No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT	55	0.11040629516621166	No Hit
GAATATAATTGACCTGCTTTCGCTCCCCCCCAACTTCGGAGGTCGACCAG	55	0.11040629516621166	No Hit
CTCCAGCACGGAGAGGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCC	55	0.11040629516621166	No Hit
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGG	54	0.10839890798137145	No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC	54	0.10839890798137145	No Hit
GAATGAGGAATCCCCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCA	54	0.10839890798137145	No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG	52	0.10438413361169101	No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG	52	0.10438413361169101	No Hit
GCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAA	51	0.10237674642685081	No Hit
CCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCAC	51	0.10237674642685081	No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT	50	0.1003693592420106	No Hit
CAATTATTGCTTCGGCAATCGACTGTTCGTCTCTCCCACTCACTATCAGC	50	0.1003693592420106	No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA	50	0.1003693592420106	No Hit
GTCTGATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCAT	50	0.1003693592420106	No Hit
ATCCTGGAGTTCTTCTTTCTTCTCTTTGGTTATCGTTAGTTGCGATTCCG	50	0.1003693592420106	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGC	10	0.007075034	144.97089	145
CGTCAGA	45	1.1490238E-4	64.822	2
GCGTCAG	50	1.925206E-4	58.39879	1
AGACCCC	50	1.9936467E-4	57.988354	6
CTTCTAG	40	0.0058561387	54.748863	1
TCTAGTG	40	0.0059743263	54.473465	3
GTGTAGC	40	0.0060220947	54.364082	7
TGTAGCC	40	0.0060220947	54.364082	8
CTAGTGT	40	0.0060220947	54.364082	4
GTAGCCG	40	0.0060220947	54.364082	9
ACCCCGT	55	3.1925077E-4	52.716682	8
ATAGACT	55	3.1925077E-4	52.716682	7
CAGACCC	55	3.1925077E-4	52.716682	5
TAATAGA	55	3.1925077E-4	52.716682	5
AGACTGG	55	3.1925077E-4	52.716682	9
GACCCCG	55	3.1925077E-4	52.716682	7
CCTGTTC	45	0.0095174685	48.420856	3
ACAGCGA	45	0.009593462	48.323627	6
ATGGGCA	45	0.009593462	48.323627	5
GCGACCA	45	0.009593462	48.323627	9

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content