FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_Negative_Control_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_Negative_Control_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCACAACTGTTCGTACTGCTAACTAAGCAATAAATAAGTTCTAATCTG15.263157894736842No Hit
ATCTTTATTCACTCGAGAGAAAAAACTTGAACATCACAGCTTAGCGTTCT15.263157894736842No Hit
CTCTTGACTGATGATCCGCTTGTTCTCTTAAATGTGAATCCACCAAAACT15.263157894736842No Hit
GGCAAGGGCAGTGAGAGAACGCAGGAGAGGGATCCTTTCCTCCCTGCAGC15.263157894736842No Hit
GATCTGAATTTCGTCAAGAGGGCGAGTCAGCGATTGGGTGCTATGCAGGA15.263157894736842No Hit
CAAGTATTCTCATTCCTGATCCCCTCACATTCACAGTAAATGAGGAGAAC15.263157894736842No Hit
GAATGGACCAATAACAAATACAGTTCATTATCCAAAAATCTACAAAACTT15.263157894736842No Hit
GCCACTTCCAGAAGCTTCTCCCACAACACCCTATTTCATGACTCACATTC15.263157894736842No Hit
CCAAAATACCAACTCATAATAAAATTTAGGTTAGTGAAAAAATAATACAA15.263157894736842No Hit
GTAGTATATAGTCAAGGTGATAGTTCCGAAATAAGAATAACAATCCGGTA15.263157894736842No Hit
CAGTAGAAGGTGAAATCATTAGATATTAGCGAGGTGCAGTGTAATAAAAA15.263157894736842No Hit
ATTGGGGAGTTGAACCTATCGACAATGTGATGGGAATGATTGGGATATTG15.263157894736842No Hit
GTCAGTTTGTCTCTCTCTCCAGCTCTGTGTCAAATAAATAAATGACATCT15.263157894736842No Hit
CTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAATATCCCAATCAT15.263157894736842No Hit
GTATTGACCAACACTGATTCAGGACCATTAATCTCCCACATCATTGACGA15.263157894736842No Hit
ATGGCACAGGGTGTGGGGACCAGCCAGGCGGCAGAAGCACAGCTCGGGGG15.263157894736842No Hit
ATTTCCAGTTATCTTCTTGGAATTGATTTCTAGTTCTATACCACTGTGGT15.263157894736842No Hit
ATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAAGAGC15.263157894736842No Hit
CCTGTCTCCTCCCTGCTGTCCGCCTTCTGTACCTGGCCCTCCCCTTCTAC15.263157894736842No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)