FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_Chang_08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_Chang_08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651323
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT22860.35097793260793797No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC17100.2625425480138119No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA14590.22400560090769095No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA13030.20005435091344845No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA12590.19329887014584163No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG11620.1784061057263447No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG10250.1573719951544779No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA9950.1527659855402005No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT9530.14631757208021212No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT9320.14309336535021794No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA9290.1426327643887902No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC8970.1377196874668943No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT8850.13587728362118334No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA8510.13065713939166895No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGGACAATT8360.12835413458453024No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT8140.12497639420072683No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGA8090.12420872593168059No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGGAGTA8070.1239016586240621No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCGGGCACTGAAG8000.12282692304739738No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTTTGG7980.12251985573977887No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC7940.12190572112454189No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC7890.12113805285549564No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG7350.11284723554979635No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT7120.10931596151218367No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT7090.10885536055075591No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCCGGAGT7080.10870182689694668No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAATTGTGT6990.10732002401266345No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA6990.10732002401266345No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT6720.1031746153598138No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAC6590.10117867786029358No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA151.2261688E-4145.107962
AGAGCTA100.007089177145.007664
ACCCTTA100.0070924326144.9854145
TCTGACC301.1019438E-7120.82118145
AGCACGC4200.0115.8298651
GAAACAC3350.0112.5432744
GCACGCA4350.0111.74982
CACGCAC4350.0111.689733
AAACACG3650.0103.285215
ACACGTG1350.0102.026787
AACACGT1350.0102.026786
GAAGCGG1100.092.412431
GCTGCCC406.1187166E-790.615875145
ATTCACC259.389019E-486.99125145
AACACGA2900.082.49176
GCGGTGC1100.079.2106551
ACGCACT6300.078.258114
CGCACTT6400.075.896625
ACACGAG3250.073.607987
AAGCGGT300.001929731772.553982
Produced by FastQC (version 0.11.5)