FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_Chang_07_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_Chang_07_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6124
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT270.44088830829523185No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT240.3919007184846506No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA150.24493794905290658No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT150.24493794905290658No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT140.22860875244937948No Hit
AGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGGAA140.22860875244937948No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT140.22860875244937948No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA130.2122795558458524No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA130.2122795558458524No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC130.2122795558458524No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT130.2122795558458524No Hit
GAAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGG120.1959503592423253No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT120.1959503592423253No Hit
TGACTACCCTGGTCAAGACCGCCTAAACAGACTAAAGAGAAAATTAGAGT110.17962116263879815No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA110.17962116263879815No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC100.16329196603527107No Hit
CTTTATGAACTGGTCAGTTTTCATAACCTCCTGGTCTCTCATTTGTTTGA100.16329196603527107No Hit
CTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACACTG100.16329196603527107No Hit
AAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGGA100.16329196603527107No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA100.16329196603527107No Hit
TTTGAAAGCATAATTCCTCAGAAGATGGCTGGTCAGTACAGTGGATTTGC90.14696276943174394No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT90.14696276943174394No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT90.14696276943174394No Hit
CTCTACAGCAGAGGGGCCCAGCATTTTCTTCAGCTTTCTTTCAAGCGCCA90.14696276943174394No Hit
GTATGTCAAGTATGATGGAGACCCATGGGCTGACAGTGATGCCCTAGCTT90.14696276943174394No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA80.13063357282821686No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGGAGTA80.13063357282821686No Hit
ATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACACTGAT80.13063357282821686No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT80.13063357282821686No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA80.13063357282821686No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA80.13063357282821686No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTATT80.13063357282821686No Hit
CCACTGTACTGACCAGCCATCTTCTGAGGAATTATGCTCCCAAATGCTTC80.13063357282821686No Hit
GGTCAAGACCGCCTAAACAGACTAAAGAGAAAATTAGAGTCAAGAATAAA80.13063357282821686No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA80.13063357282821686No Hit
TAACAGGCCCTTGTGAATCTAATGGGGACAAAGGGAGTGGAGGCATCAAG70.11430437622468974No Hit
GTATGCAGAAACCAACCTGCTGCAACGGCACTGAACACAACAATAACCTC70.11430437622468974No Hit
ATCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAA70.11430437622468974No Hit
AAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAATGCAGA70.11430437622468974No Hit
GCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTC70.11430437622468974No Hit
AACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACA70.11430437622468974No Hit
GCTCCCAAATGCTTCAAATGCATCCCATTGGAACATGTCCTCTTTTCCTA70.11430437622468974No Hit
GGATGGGACTGTATGTCAAGTATGATGGAGACCCATGGGCTGACAGTGAT70.11430437622468974No Hit
GGCTTTGGTCATGTCATATCCTTGTATTGATGATTCCTGTTCAACAATTG70.11430437622468974No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT70.11430437622468974No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT70.11430437622468974No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA70.11430437622468974No Hit
GCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACACTGATGATAG70.11430437622468974No Hit
GTTGAGTAGTAAAGCAGTATAGTATGATTATCCAATCCATCGTCATTTAA70.11430437622468974No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG70.11430437622468974No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT70.11430437622468974No Hit
CCAAAGAACTGGTGCAAAACACTTATCAATGGGTGCTAAAAAACTTGGTG70.11430437622468974No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT70.11430437622468974No Hit
TTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGTTTCTTTGGC70.11430437622468974No Hit
TGCTAATACTGCATTACCCATTGATCTATTCCTTTCTTCATCTTCAATTT70.11430437622468974No Hit
GTATTAGTAGTTGAAAACTTGGCTAAAAATATATGTGAAAATCTAGAACA70.11430437622468974No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTG100.006752917146.181812
AACACGT100.006752917146.181811
CACTTTC151.1719272E-4144.983617
GCACTTT151.1719272E-4144.983616
CACGTGC100.006922572144.98363
ACGCACT100.006922572144.98364
CACGCAC100.006922572144.98363
TAACTGT100.006922572144.98364
AACTACA100.006922572144.9836145
CGCACTT100.006922572144.98365
ACGTGCA100.006922572144.98364
ACTTTCT258.946709E-486.990168
CTTTCTT300.001845324372.4918069
GCTTTCA300.001462348924.16393350-54
ACAATTG300.001462348924.16393335-39
CTTTCAT456.666118E-419.33114850-54
GGGGGGG3401.6370905E-1110.66056145
Produced by FastQC (version 0.11.5)