FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_Chang_06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_Chang_06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36408
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATAAACAGTGAAGATGAACATCTCTTGGCACTTGAAAGAAAGCTGAA720.1977587343441002No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC720.1977587343441002No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG710.1950120852559877No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT700.19226543616787517No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT540.14831905075807514No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACCA530.14557240166996266No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT520.14282575258185015No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT510.14007910349373764No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT510.14007910349373764No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT490.13458580531751263No Hit
GTATAATGGAGACCCATGGGCTGACAGTGATGCCCTAGCTTTTAGTGGAG470.12909250714128764No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT460.1263458580531751No Hit
GTACTTCCTTCATTGTTTCTTTGGCCTGGATTTCTTTTTGGTAACTGTGT450.12359920896506263No Hit
GTACTGACCAGCCATCTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG450.12359920896506263No Hit
TACCATTCCTATGCAAACAAAATACTAAGAACACAAGAAAGTGCCTGCAA440.12085255987695012No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATACTAAGAACAC440.12085255987695012No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA440.12085255987695012No Hit
AGTAAGATGTTTGCCTTTTCCCCTGGTTTCAGCTTTTCAAGTTCTTCAAT410.11261261261261261No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC400.10986596352450011No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG400.10986596352450011No Hit
GTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCAAGAGG390.10711931443638761No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC380.1043726653482751No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA380.1043726653482751No Hit
CCATTATACTTGACATACAGTCCCATCCCCATCCTTTCAGTCTTAGACAT370.10162601626016261No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATC370.10162601626016261No Hit
CCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGCAAAACACTTA370.10162601626016261No Hit
CCTTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACT370.10162601626016261No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAAG100.0070630163145.03
GAAACAC100.0070630163145.04
AGCGGAG100.0070630163145.01
CAACGCA100.0070630163145.09
CACGTGC256.380895E-6116.01
ACACTGC203.8358706E-4108.756
TGCTACA203.8358706E-4108.752
ACGTGCA203.8358706E-4108.752
TAGTAAC301.5787418E-596.6666642
GTAACAA301.5787418E-596.6666644
CTACACT259.313726E-487.04
TAACAAG353.3928824E-582.857145
GCTACAC300.001920739372.53
CATATAC451.1785276E-464.444443
CTGACAC753.6608253E-758.0000049
AGTAACA501.984504E-458.03
ACTGACA651.0727386E-555.769238
ATATACT553.177904E-452.7272724
TATACTG604.882128E-448.3333325
CACTGCT450.00956525648.3333327
Produced by FastQC (version 0.11.5)