FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_Blank_Space_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_Blank_Space_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1733840
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT174121.0042449130254232No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC127150.7333433304111107No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA93310.5381696119595811No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG92540.5337286024085268No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA83420.481128593180455No Hit
ACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGA83380.48089789138559497No Hit
GTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATG73800.4256448115166336No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA70540.4068426152355465No Hit
TATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTT70440.4062658607483966No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA68810.3968647626078531No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC65590.378293268121626No Hit
CTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAA64110.3697573017118073No Hit
GGGTTACATAGAACTGATTTTTAGCATGTGCTCCAACTGAGCTTTGAGGA58040.3347483043418078No Hit
ACCCAAATGTGCTCTGATGATGAATGGATAAACAAAACATGGTGTATACA54540.3145618972915609No Hit
ATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAAC53530.3087366769713469No Hit
GTTTAGACCTGTACCCAGCAACTGATTACACAGTGAATGTCACTCTCCTG52860.30487242190744246No Hit
GTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCCAAATG51710.29823974530521846No Hit
GTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATA50000.28837724357495503No Hit
AAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTC49680.28653162921607533No Hit
TTACCATACAGATGACTGTGCTTTTTAAAGTAACAGACTCTGAACTCCAA48400.2791491717805565No Hit
GTTGTACAACCTCCAAAACTTTTTCATCTTGTGAAACTCTGTTGTCCTTG47320.2729202233193374No Hit
GGTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATC46050.26559544133253354No Hit
GTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGAATAGTGGTTGG45960.26507636229409864No Hit
ACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGAATAGTGGTTGGAA45940.26496101139666867No Hit
GATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAA45190.26063535274304434No Hit
GCTTAACAGCTATCATTTTAGGATATAAAAAGCGGTTTTCTTTTTATCTT43960.2535412725511004No Hit
CCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTG43410.25036912287177593No Hit
GTAGAATAGTGGTTGGAAGGGGCTGGGGAAAGAGGAAAATGGGGAATTGT42810.24690859594887646No Hit
TATTAAAGGTCATTTCCTGGGCAAATTCCTTCTGATACCATCTCTGGCCC42630.24587043787200666No Hit
GTCTACAGCTCTGTGGTGACTTCATTCATCCGAACGCTGCCATGCCCGAT42600.24569741152586166No Hit
GTCATATTAAAGGTCATTTCCTGGGCAAATTCCTTCTGATACCATCTCTG41930.24183315646195724No Hit
GATGATGAATGGATAAACAAAACATGGTGTATACATACAATGGCATATTA40950.23618096248788817No Hit
CCCCAGAAGTGTGCCTAGATCTGCACCGAGGCACCAACTACACCATGAGC39470.22764499607806948No Hit
GATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAG38800.22378074101416506No Hit
TTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGAAACTCTGTTGTCCT38710.22326166197573016No Hit
AAGTAGAATAGTGGTTGGAAGGGGCTGGGGAAAGAGGAAAATGGGGAATT38520.22216582845014532No Hit
GTCTTTGTACTTCTCCATCTCAAGCAGGTTTCGTTAAATACTGAAATGTT37780.217897845245236No Hit
GTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAA37680.2173210907580861No Hit
CTCCTGAGGCACTCACGACAAAATGACGATTACACATAACTGAGAAGCCG36600.21109214229686704No Hit
ACATAGAACTGATTTTTAGCATGTGCTCCAACTGAGCTTTGAGGAGATAA35890.20699718543810272No Hit
GTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCT35370.2039980621049232No Hit
TCTCAGGAGCCTGGCTGTGGGCAGTCATATTAAAGGTCATTTCCTGGGCA33900.1955197711438195No Hit
CTCTTAGAAAGGTAGTCAGGACAAAATACTAATGCGAGTTAAATGTGGCT33900.1955197711438195No Hit
CTATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGT33860.19528906934895954No Hit
CCTCTGTATCAGCGGTCTTTGTACTTCTCCATCTCAAGCAGGTTTCGTTA33830.19511604300281457No Hit
CTCTAAGTACCTTATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAA32630.18819498915701563No Hit
ACAGATGACTGTGCTTTTTAAAGTAACAGACTCTGAACTCCAATGTTGAT32060.18490748858026115No Hit
GGCTACAGCATGGATGAACTTGGAGATCATATGTGAAGTAAAATAAGCTA32030.1847344622341162No Hit
GTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATCTCCAA31040.17902459281133207No Hit
GGTCTAAACATACCACTGGAGCTCGCTCCCTTGTTGTGAAGTTAAATGAC30920.17833248742675217No Hit
AAACAAGGCATTGGGTTACATAGAACTGATTTTTAGCATGTGCTCCAACT30700.1770636275550224No Hit
GTTGTAGCACACACCTGAAGGGGGGGGGTATGAAAGGGTCCTGTGGTCGT30410.17539103954228763No Hit
TTTCAGATCCACTTGATAAAAAAGGGCTATTTCCTTTATTCAGTTCTTTG30290.17469893415770774No Hit
GTATTGGGGTGAGTCACAGTTCTCAAAACAGAGAGTAAATCCTGTCACTT29900.1724495916578231No Hit
CCTTTATTCAGTTCTTTGAACTCTTTTCCTCTGTTTCCTCATTTAATCAA29430.1697388455682185No Hit
GGATGGAGCCATAGCAGAATCCTCCATACCAAGAGCTTTTCAGATCCACT29350.1692774419784986No Hit
CTCCTGGGATCTTCTGAGCAGCAGTCAGTGCAAATAACGATGACAACCGC29320.16910441563235362No Hit
ATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGTAA29250.16870068749134867No Hit
ATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTA28810.16616296774788908No Hit
ACACATAACTGAGAAGCCGTCATGAGGAACAATTCTTTTTAGGTCTCGAG28740.16575923960688413No Hit
GAGTAATCAGATTCCTAGAAACACAAAGTAGAATAGTGGTTGGAAGGGGC28520.16449037973515435No Hit
GTGCTACAACAGTGGGAAGCAATAGGGTTCCTCTTCCTCGGGGCCCGTCT28480.16425967794029436No Hit
CCTCCAAATAGCTAAAAGGTTAATCAACCCAAATGTGCTCTGATGATGAA28470.1642020024915794No Hit
GTATTGGAGGGCACCAGACTGGCTCGATCAGTAGAGCGGGCAATGCTTGC27940.16114520370968485No Hit
TTCTAAGAGAGAAGACATGACAGGTACGACCACAGGACCCTTTCATACCC27550.1588958612098002No Hit
CCCCAGATTTGAGGAGCGACAATGAGAAGATGAGAGAAAAGTGAAATTGG26970.15555068518433074No Hit
CTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTA26840.15480090435103586No Hit
TAACTGAGAAGCCGTCATGAGGAACAATTCTTTTTAGGTCTCGAGTATGT26840.15480090435103586No Hit
CAATAACACAAAACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTA26290.15162875467171136No Hit
CAGTAAAACAGATCATCGGTAACATTTCAGTATTTAACGAAACCTGCTTG25800.14880265768467676No Hit
ACTCTAAGTACCTTATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACA25650.14793752595395193No Hit
GATCTGCACCGAGGCACCAACTACACCATGAGCATTTCCACCGCCCCTCC25490.14701471877451205No Hit
CTTCTAATTGTGGTAAAATACAATAACACAAAACTCACACCTTAATCATT25300.14591888524892724No Hit
GTTTTACTGCTGGTGCGGTTGTCATCGTTATTTGCACTGACTGCTGCTCA25250.1456305080053523No Hit
CCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCC25030.14436164813362248No Hit
ACATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGAATAG25000.14418862178747752No Hit
GTATGTATACACCATGTTTTGTTTATCCATTCATCATCAGAGCACATTTG24920.14372721819775758No Hit
AGTAGAAACAAGGCATTGGGTTACATAGAACTGATTTTTAGCATGTGCTC24800.14303511281317768No Hit
GAATAAATACATCTCCTCTGTATCAGCGGTCTTTGTACTTCTCCATCTCA24620.14199695473630786No Hit
GAATAAAGGAAATAGCCCTTTTTTATCAAGTGGATCTGAAAAGCTCTTGG24570.14170857749273288No Hit
TCTGAAGACCAAGTAATATGCCATTGTATGTATACACCATGTTTTGTTTA24560.14165090204401792No Hit
CACCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCAT24490.14124717390301297No Hit
CTGATGATGAATGGATAAACAAAACATGGTGTATACATACAATGGCATAT24250.13986296313385319No Hit
ATTTTAAATATCCGGAAGGAATGTAACCAGCGCTCACATACACGCTAGCC23960.13819037512111845No Hit
ACACAAAACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTT23940.13807502422368848No Hit
GTATCAGCGGTCTTTGTACTTCTCCATCTCAAGCAGGTTTCGTTAAATAC23650.13640243621095371No Hit
TACATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGAATA23240.1340377428136391No Hit
GTGAATGTCACTCTCCTGGGATCTTCTGAGCAGCAGTCAGTGCAAATAAC23240.1340377428136391No Hit
CTCCTATTCTTCCACAAAGGATGGAGCCATAGCAGAATCCTCCATACCAA23100.13323028653162922No Hit
GTACAAAGACCGCTGATACAGAGGAGATGTATTTATTCTACATTCGGGGC22970.13248050569833433No Hit
CTACAGGTATGGCCACAGTTGACAGAGTATTGGGGTGAGTCACAGTTCTC22870.13190375121118442No Hit
CTTCAGGTGTGTGCTACAACAGTGGGAAGCAATAGGGTTCCTCTTCCTCG22740.13115397037788953No Hit
ACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACA22730.13109629492917454No Hit
GAATGTAACCAGCGCTCACATACACGCTAGCCACATTTAACTCGCATTAG22480.12965440871129977No Hit
ATACTAATGCGAGTTAAATGTGGCTAGCGTGTATGTGAGCGCTGGTTACA22070.12728971531398514No Hit
TCATTTAACTTCACAACAAGGGAGCGAGCTCCAGTGGTATGTTTAGACCT22070.12728971531398514No Hit
CATACACGCTAGCCACATTTAACTCGCATTAGTATTTTGTCCTGACTACC22060.12723203986527015No Hit
CCTCCATACCAAGAGCTTTTCAGATCCACTTGATAAAAAAGGGCTATTTC21960.12665528537812024No Hit
GCTAAAAGGTTAATCAACCCAAATGTGCTCTGATGATGAATGGATAAACA21690.1250980482628155No Hit
GTCCCACACCAACCAGGTGCTTGAAGCAGGCTGTGTGGAAGGGCAGGCAT21600.12457896922438057No Hit
GTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAG20970.12094541595533614No Hit
ATACAGATGACTGTGCTTTTTAAAGTAACAGACTCTGAACTCCAATGTTG20610.11886909980159646No Hit
TCCCAGGAGAGTGACATTCACTGTGTAATCAGTTGCTGGGTACAGGTCTA20600.11881142435288146No Hit
GAATAGTGGTTGGAAGGGGCTGGGGAAAGAGGAAAATGGGGAATTGTTCA20500.11823466986573156No Hit
GCTCTGTGGTGACTTCATTCATCCGAACGCTGCCATGCCCGATTTACCTG20460.11800396807087159No Hit
ATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGAAACTCTGTT20430.11783094172472662No Hit
GTGCAAATAACGATGACAACCGCACCAGCAGTAAAACAGATCATCGGTAA20120.1160430028145619No Hit
TCCCAGGACCCTGGGATCATGACCTGAGCTGAAGACAGATACTTAACTCT20090.11586997646841693No Hit
CTTTTTATCTTTGTGTAAGGTAAGCTTCCAAGTTTTGGTGGAAATTCCAG20060.11569695012227195No Hit
GTCATGTCTTCTCTCTTAGAAAGGTAGTCAGGACAAAATACTAATGCGAG19960.11512019563512203No Hit
GTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGAAACTC19760.11396668666082221No Hit
CTCCATGCCGGCCATCATCGGGTTCCAGACAGCTGTTTCAAGTTCTGGGT19590.11298620403266738No Hit
GTACTGTGTTCCCATTCGGTACCCCGAAGAACCCCGAAGAACCCCGCGGT19450.1121787477506575No Hit
GGTCAAAGGTGGTACCTGAATAATTTTTATCATGCAACGTCATTTAACTT19370.11171734416093758No Hit
TTTGAAATGTGCTCCTGCTTAACAGCTATCATTTTAGGATATAAAAAGCG19350.11160199326350759No Hit
GCATATTACTTGGTCTTCAGATGGATGGAAATCCTGACACGGGCTACAGC19300.11131361601993263No Hit
GCCAAGCACTGTTCCCTGAGAATAACGGACTCCTGAGGCACTCACGACAA19260.11108291422507266No Hit
CATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGAATAGT19230.11090988787892771No Hit
CATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGAAACTCTGT19200.11073686153278271No Hit
GTGTAAGGTAAGCTTCCAAGTTTTGGTGGAAATTCCAGCGTGATTCCATT19130.11033313339177779No Hit
GCTGTAGCCCGTGTCAGGATTTCCATCCATCTGAAGACCAAGTAATATGC19000.1095833525584829No Hit
GCTGTAGACGGGCCCCGAGGAAGAGGAACCCTATTGCTTCCCACTGTTGT18930.10917962441747797No Hit
CTTCTGAGCAGCAGTCAGTGCAAATAACGATGACAACCGCACCAGCAGTA18700.10785308909703317No Hit
GGCTAACATTACCATACAGATGACTGTGCTTTTTAAAGTAACAGACTCTG18690.10779541364831818No Hit
CTCCAATGTTGATTAAATGAGGAAACAGAGGAAAAGAGTTCAAAGAACTG18620.10739168550731323No Hit
GTGGTGACTTCATTCATCCGAACGCTGCCATGCCCGATTTACCTGCAATG18600.10727633460988326No Hit
TACCTGTCATGTCTTCTCTCTTAGAAAGGTAGTCAGGACAAAATACTAAT18570.10710330826373829No Hit
ATACAGAGGAGATGTATTTATTCTACATTCGGGGCCAGAGATGGTATCAG18480.10658422922530336No Hit
GTTCAAAGGCGCGCAAAGTAGAGCAGTACTGTGTTCCCATTCGGTACCCC18430.10629585198172842No Hit
CTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGT18220.10508466755871362No Hit
GTTCAAGGACAACAGAGTTTCACAAGATGAAAAAGTTTTGGAGGTTGTAC18180.10485396576385365No Hit
TGCTACAACAGTGGGAAGCAATAGGGTTCCTCTTCCTCGGGGCCCGTCTA18140.10462326396899368No Hit
TAATATGACTGCCCACAGCCAGGCTCCTGAGATGTGCCTGGACCTGCGTC17690.10202786877681908No Hit
GTAATATGCCATTGTATGTATACACCATGTTTTGTTTATCCATTCATCAT17680.1019701933281041No Hit
AGCTTATTTTACTTCACATATGATCTCCAAGTTCATCCATGCTGTAGCCC17660.10185484243067412No Hit
GTTCCAGACAGCTGTTTCAAGTTCTGGGTCAAAGGTGGTACCTGAATAAT17500.10093203525123426No Hit
GTCTTCAGATGGATGGAAATCCTGACACGGGCTACAGCATGGATGAACTT17480.10081668435380428No Hit
GTAAAATACAATAACACAAAACTCACACCTTAATCATTCTAAAGTGTACG17440.10058598255894431No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT100.00709542144.9719145
CGCCGTT100.00709542144.9719145
GTCACCC500.0130.4747145
AGCGAAA6350.0121.279961
CGACGGC203.8695242E-4108.72891145
GAGCGAA1100.099.073041
CCGACGC2150.097.77173145
GAGTGTT406.126447E-790.60743145
GCGAAAG8600.089.47992
TCTGACC2050.088.39749145
AAGTGTG7400.084.240427
CGAAAGC9550.080.497393
CCAGAAG7900.079.867753
GTAGAAA7700.075.4253542
AGTGTGC8850.075.3526158
CATAATG1750.074.556977
GTGTGCC9000.074.096749
CTACAGG4450.073.469891
CAGGTAT4350.073.333924
TCCGCAA406.6709705E-572.48595145
Produced by FastQC (version 0.11.5)