FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_Blank_Control_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_Blank_Control_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATTGAAGACTCTGATTCGTTAGACAACATTAAAAAACATTTTTTTC24.0No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC24.0No Hit
CAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCATCATGCTG12.0No Hit
TTTCATGCCCTCTTTTAAAGCATGATTAGATTTACGACCGCGAGTTGAAG12.0No Hit
ATTTTTACCTTTTCTGCTCTGGTTTCTCTCCATCTTTGTGGTTTTATCTA12.0No Hit
ATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGAT12.0No Hit
AGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATC12.0No Hit
ATTTTGTGTGTGTTTGTCGGCAGGAGGAGTGAAATTTGCTTCCTGCTAGC12.0No Hit
CTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCT12.0No Hit
GGTCAGAGGGAGAGAGAGTATCCTCCAGCAGCATCCCTGCTGAGTGTGGA12.0No Hit
TTTGTTCTTGGAAAAAAAACCCATTTTTATTTTTTGAGACTACTAAAAGG12.0No Hit
TATGTAGGGTAATTACACAAAATACAAAAAAAATTTAGGTATTATAAAAA12.0No Hit
GCTGGAGGTGGCGCCCGCGCCCACTTCTCCGGTCACGTATCCCGTGTCGG12.0No Hit
TAAATAAATAAATAAAATATTAAAAATATATGTAAGGATTTAATATTCTA12.0No Hit
GAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTATGAGCAATTACA12.0No Hit
TAGATAACATTCTCTACAAATCGTTACTGTGACCCTGAAGTAAGGAAGTT12.0No Hit
ACCCAGGACACTGCAGCGGACACCCTTGGGTAAAGACCGTGAAACGGATG12.0No Hit
TGTGTGGGAACCGGGCAGCTCCCATGGGTCTACTAAGCTGGGTCAAGAGC12.0No Hit
CAACCGCAACCAATGCAGTGAAGATCCCCTAAACCCACCTCTGATCCCAT12.0No Hit
GTATAAGAGTCGTGTTATTTTATTTAACAATACAATTAAAAAAATAACAT12.0No Hit
ATAAGAATGTAACTGGATTTTCAGTTCAGTGAATTTTATCATACTCTTCC12.0No Hit
GCTTGCTTCCCCACAAAGAAAACCTAGCAACCAATTCTCTTAACTTCCCA12.0No Hit
GAACCCAGAGAATCCATAATACCATAGAAAGATTCTTAGCTGCCGAACAC12.0No Hit
CAAATAGAATATACTCCTCTAAAGTCCCCCTAAAACCTTCTCCACCTAAC12.0No Hit
CACTACAACAGGTCCCTCCCCCAGAAGATCAGCAAGAACATCCAGCCAAG12.0No Hit
GCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGA12.0No Hit
CTTCCTAAACAACTAATGGGTCCATCTTTATGAACATTTTGTGAATTAAA12.0No Hit
GACTATGACTATAGGGTTAGCCTGAAGTATGATTCTTCCAGAAGATTGAT12.0No Hit
TGCTAAGAGTGTCCTAAAAATTGTCTAGACACAGCTTTTGTTCTGCATAA12.0No Hit
GTTTTAGTCCCCAACTTGGGAAATTTCTTCCAACATCATATTTTACCGCT12.0No Hit
GAAAATAATAATCACCATTATACACATAAAATAAGAAATCAAAAACATCA12.0No Hit
TTATTGAGTCAGATACAGTTTTCTTGGCTATCACAGGTTCTGGAGTAAAA12.0No Hit
ACTCAAGCCCATGGGACGGAGCTGCCTATTGGGATCAGCCCTTGGCCATA12.0No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG12.0No Hit
GTACTGATCCAAAATAGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA12.0No Hit
ATTTATGTAAGACTTCTTTTTGATCATGTAGATTACAACTAGCTTGCAAG12.0No Hit
GTAGAAAGGGGTCTTGACGTGGCAACCACGATCAAGCCGCTCGAGGACCG12.0No Hit
GTTTGGGGCTGGCGCCTCTACGTGTGGGCGTGGATGAGGGTCTGTAAGGT12.0No Hit
GCTGAATATGGTGTCGCGTGCCTGTAAGCCCAGCTACTCGGGAGGCTGAG12.0No Hit
CTATTCGTCAATTGCACAGCTGTAATTTGTTCACCCAACATGTTAGTTAT12.0No Hit
GGTTTGAATTTCCTCCTGTAGCTCGGAGTAGTTTGATCGTCCGAAGCCTT12.0No Hit
CAACCCTGTTCTAACTAGATTTGTAAGGTCTATTACTTCGCAAATTCGTA12.0No Hit
GTGTAGGCACCAGCAAATCGGCAGAATCACAGCTCGGCCTAGAACTGATT12.0No Hit
CTATAAATAACAAACAAATCTTATTTCATATCGTCATTAATGACTCCTTC12.0No Hit
GCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCA12.0No Hit
GCCTCGGTCTCCCTGTCTGTAAAATGAGTTTTCTTCCAGCTCCGCACTAA12.0No Hit
ATTGCAGGAAGAGCAGGTTGAAAAGAAAGATTAGGCATGTGGTTTTGGAT12.0No Hit
GTTGTAGGCTGCGTGTGAACATCCCCTCTTTCCCGCCGTTTTCGGAGGGC12.0No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAACA50.0145.03
CATTCTC50.0145.08
ACATTCT50.0145.07
ATAACAT50.0145.04
CTGAAAT50.0145.0145
TAACATT50.0145.05
ATTCTCT50.0145.09
TAGATAA50.0145.01
AACATTC50.0145.06
AGATAAC50.0145.02
Produced by FastQC (version 0.11.5)