FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_AHK1073_99_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_AHK1073_99_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100794
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA8070.8006428954104411No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG5910.5863444252634086No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT5750.5704704645117765No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG5720.5674940968708454No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG5470.5426910331964204No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA4970.49308490584757025No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT3730.370061710022422No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG3220.3194634601265949No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT3200.31747921503264087No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT3100.3075579895628708No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT3100.3075579895628708No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT3090.30656586701589383No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC3080.30557374446891683No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG3060.3035894993749628No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT3000.29763676409310075No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC2920.2896997837172848No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC2880.28573129352937676No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA2750.2728337004186757No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC2680.2658888425898367No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTC2570.25497549457308966No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACT2540.2519991269321587No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT2490.24703851419727363No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG2330.2311645534456416No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGT2220.2202512054288946No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATC2200.21826696033494056No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA2190.21727483778796355No Hit
ATACTGAGGACCCAGATGAAGGAACAGCAGGAGTAGAGTCTGCAGTATTG2160.21429847014703254No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC2030.2014008770363315No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA1990.1974323868484235No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG1960.1944560192074925No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC1960.1944560192074925No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACC1940.1924717741135385No Hit
GACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACACATCAG1920.1904875290195845No Hit
TCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCA1890.18751116137865348No Hit
GTTCATTGATGCTTAATGCTGGTCCATATCTTTTGTCTTCTTTGCCGAGG1880.18651903883167648No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT1750.17362144572097546No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1720.17064507808004445No Hit
TCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAATGTATTGATA1720.17064507808004445No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG1700.16866083298609044No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA1630.16171597515725142No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA1620.16072385261027441No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG1590.1577474849693434No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC1580.1567553624223664No Hit
TCGTAAGATTGCTCAGTTCATTGATGCTTAATGCTGGTCCATATCTTTTG1570.1557632398753894No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA1500.14881838204655037No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAA1410.13988927912375737No Hit
ATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACAC1400.13889715657678037No Hit
CTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGA1400.13889715657678037No Hit
TTATATGCCCATACGTCTTGTATTTGGTCATCAATCTTATTATTGATCAT1380.13691291148282636No Hit
CACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAA1370.13592078893584936No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA1360.13492866638887235No Hit
GTACTAGCAATTCTGCATTATATGCCCATACGTCTTGTATTTGGTCATCA1360.13492866638887235No Hit
TCTCTATACTTTCTCCTATTATAGGTCCCGTTCCGAATTGTTTCCATGCA1340.13294442129491835No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA1330.13195229874794134No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA1320.13096017620096434No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT1310.12996805365398734No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC1300.12897593110701033No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA1300.12897593110701033No Hit
CTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACACATC1270.12599956346607935No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG1260.12500744091910232No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT1230.12203107327817132No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA1220.12103895073119432No Hit
TTACCAACACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCT1220.12103895073119432No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC1210.12004682818421732No Hit
ACACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTA1200.11905470563724031No Hit
GGAGTAGAGTCTGCAGTATTGAGAGGATTTCTAATCCTCGGCAAAGAAGA1190.11806258309026332No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA1190.11806258309026332No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT1170.1160783379963093No Hit
CTTCTAAGTGTGTGCATATTGCAGCGAACTTATTTGTTTCAATTTTCGGG1160.1150862154493323No Hit
GTATTGAGAGGATTTCTAATCCTCGGCAAAGAAGACAAAAGATATGGACC1160.1150862154493323No Hit
GGTCCATATCTTTTGTCTTCTTTGCCGAGGATTAGAAATCCTCTCAATAC1140.11310197035537829No Hit
TTGTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTG1140.11310197035537829No Hit
ATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTT1130.1121098478084013No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG1130.1121098478084013No Hit
GCTTAATGCTGGTCCATATCTTTTGTCTTCTTTGCCGAGGATTAGAAATC1130.1121098478084013No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA1120.1111177252614243No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG1110.1101256027144473No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG1110.1101256027144473No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC1110.1101256027144473No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG1100.10913348016747028No Hit
ATCTTACGAAAGGGGAGAAAGCTAATGTATTGATAGGGCAAGGAGACGTA1100.10913348016747028No Hit
CTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTC1080.10714923507351627No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGCCC1050.10417286743258528No Hit
ACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTTAA1030.10218862233863128No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC1030.10218862233863128No Hit
ATCTCGCACTCGCGAGATACTGACAAAAACCACTGTGGATCATATGGCCA1020.10119649979165425No Hit
GTGGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAA1010.10020437724467726No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC1010.10020437724467726No Hit
TTCCAGGACATACTGACGAGGATGTCAAAAATGCAATTGGGGTCCTCATC1010.10020437724467726No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGGA100.0070427465145.266041
GCAGGTA950.0145.266042
ACAGCGC100.007084731144.97766145
GGTACTG1000.0137.728785
CAGGTAC1050.0131.23543
GTACTGA1050.0131.170276
GCGAAAG750.0116.212832
AGCGAAA750.0116.212831
TCTGACC256.4414053E-6115.98213145
AGGTACT1250.0110.183034
GTCTAAG203.8278272E-4108.9495241
CTGATCC1300.0105.945229
CGAAAGC850.0102.387993
GCTCTAT259.2941104E-487.159621
TCCAGAT259.2941104E-487.159622
TCTATGT259.3493523E-487.0297853
CTATGTT259.367821E-486.98664
AAATATA1300.083.640965
TACTGAT1750.078.702167
AAAGCAG1150.075.6405265
Produced by FastQC (version 0.11.5)