FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2254_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2254_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATATGGTATATAGTATTTGTAGCTCACTGAAAAAACACAACATTTCT10.9615384615384616No Hit
CACTTATGCAGATCATGAGATAAGCGACTTTCATAATTTTTTTGCATTTA10.9615384615384616No Hit
CTCTAACTCTCAGGGAGAACTCATCTCGGGGCAAGTTTAGTGATTAGATC10.9615384615384616No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC10.9615384615384616No Hit
CCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGC10.9615384615384616No Hit
GTCCCTACCTACTCGGGTCAACATTGATCATTTAGGGAATGTGTAGGGGA10.9615384615384616No Hit
GCTCAGCATGACTCGTAGTCCTCAATCACCACGACCATGACATCAAAACA10.9615384615384616No Hit
ACGTTAAACTACAACGGTGGCTTAAACTGCAACGTCTCGCCATTCTGGAG10.9615384615384616No Hit
CCTCTGGATGAGCTGGACGAGTGAGTGATGGATCAGCAGGGAGAGCGGGG10.9615384615384616No Hit
ATATTAGGGAGCAATACTTCAGAACGCAATAACGAAATGAGCATTAAAGG10.9615384615384616No Hit
CAAAGGCTAACCCTCACCACATCTGCCCTGATGGCCTGCCACTTGCAACT10.9615384615384616No Hit
AGAGCAAACGCGCCAGCGTCGCATCCGGTATTAATTGATATGAAAAAAAC10.9615384615384616No Hit
GAGAAAGAAAGAGTTAAGTGATAAGGGCTTCCTGAAGGAGCTGTGACCTG10.9615384615384616No Hit
ATCCCAACGTTGTATATACAGTTTTGAAAAGTGGGACCTGGGTGCATAGT10.9615384615384616No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC10.9615384615384616No Hit
ATTCAGATGTGTCAAATTTCTTCATTATGTCAGCTAACACTATATACTTC10.9615384615384616No Hit
CCATTAAAGACGAAGAAAGCAACATGCAACAAGGTCAGAGCTGTGAGCAA10.9615384615384616No Hit
GTTTATAGTGATACAGGCCTAACTCAAGAAACAAGAAACGTCTCAAATAA10.9615384615384616No Hit
GAAGCACAGGTCGGGGTGGCAGTGATTTGGTAGCGGCGCTATCAAATGCT10.9615384615384616No Hit
TACTAACGTATGCTTCCTAGAATGAGAAGGAAGAAAATGAATACAGAAGT10.9615384615384616No Hit
CCGGAATGCTACTGCCCCCCCGATTTCCCATCCGCCCTCTACTGTGACAG10.9615384615384616No Hit
ATATATAACCGTAGGGAAAATACAGACACAGTTATGTCATCTTAATATAT10.9615384615384616No Hit
CCTCTAGCACATACACCAGAGCTGTGATAAGCATTGCCTGGCATTGTGAC10.9615384615384616No Hit
AGCATGGGGAGCCCCTTTCTCCCTCTTCTACTGCCTGCTGCTCCTTCCAC10.9615384615384616No Hit
GTAGATAATCAGTATAAGGTAGGTTAAGGTGAAGCAGCCATTGAGGAAGC10.9615384615384616No Hit
GTCAGAGAAGGACTCATTGCTATTCCGCATTGCAACTACACAACAAATAT10.9615384615384616No Hit
GGATGCGCTGTTTATCGATCCTGATGTGCGCGGCTGCGGCGTGGGCCGGA10.9615384615384616No Hit
ACACTTAGCAGATGCTCAATAAATAAAGATTTGGAAGGAAGACCATTCTA10.9615384615384616No Hit
AGTCTATGCCAACACTTCTACTGACCTGCTGTTGGGAGGAAGAGAATGGT10.9615384615384616No Hit
TCATGAAGCCCCTTGAGCATCTGACTTCTGGCTAATAAAGGAAATTTATT10.9615384615384616No Hit
CTTTTGTTCACAACAGGTCTATAGACGAAGCAGGCTTCTTGCAATCTGCC10.9615384615384616No Hit
AGAAAAGACTGTGCACAGGACTAAGGATTGCACTGAAACTAAAGACAAAA10.9615384615384616No Hit
TTAGTAGACAGCTGAACAGTCAGATGTCTGTTTCTTTAGAATTCAATCTT10.9615384615384616No Hit
ATTCAATCCACAAAAACAGACACATTCCGCATAACTTAAACTGTAGAATA10.9615384615384616No Hit
GTCTAGGAATCTTCTCACTTCAGACACTGGTTTTCTCATTTGCTTCAAAC10.9615384615384616No Hit
ACACAACGAACGGCGCTACGCCGTTAGACTTCAGAGTTCCGCATGGGGTC10.9615384615384616No Hit
GTTTACAGGTCTCTCTCAGAAATTACAGAAAATACAAGGAAGTATTCTTC10.9615384615384616No Hit
TACTTGTAGAGTTACAGTCTAATTTCTTCCTGGGTTAGCTAGCCCATATG10.9615384615384616No Hit
TACCAATCCTTCTCCAACTCTGCCAGACAAACAAGAGGAAGAAACGCTCC10.9615384615384616No Hit
ACCAATTGGGTTCATGGGCAAACCAAAATAGGAACACAGAAATACTTTGA10.9615384615384616No Hit
GCTTAACCCACTGAGCCACCCAGGCACCACCAGACTGAACATCTTTGATT10.9615384615384616No Hit
GCTCCTTCCACTTTGCTCAGTCTGTCTCTCTCCCCAGCTCTGTGTCAAAT10.9615384615384616No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA10.9615384615384616No Hit
CCACTGTGTCCTTTTGTCTCTTGCACTATCTCACCGATGTTTGGGAAGTT10.9615384615384616No Hit
GAGCCTACAGAGAGGACTTCAGGGAAAGTTTTCTTCACCCTTAAAAAAAG10.9615384615384616No Hit
ATACCTCTTCCCGTAATGTAGAATTTGGTTTGCAGATATTTGGGTGAGCA10.9615384615384616No Hit
GGGCTGACCGGAAGGATGTGCTGTCGGGCAGAACAAGAGTGGGTCTTGTG10.9615384615384616No Hit
GCAACTAACCTCTATATCATAAGATTGGCAGGTAGAAGGAATAAATACAA10.9615384615384616No Hit
CTTCTAATACTATGTTGAACAATATAGTACATTTCATGCTTGTTCCAATG10.9615384615384616No Hit
CTAAACCACTGTGCTTCGAAAAGATGGTTGATTTGATTTCAGTCCTCATT10.9615384615384616No Hit
TTGTTGGGGTTCTGGATCACATGACAATGCAAGTTCGCGAAATCCAGAAG10.9615384615384616No Hit
AGCCACGACAGAGTGAACTTGAAGACTGAGCAGACATGAAGTACCCATAA10.9615384615384616No Hit
AGACAGAACAGTTCCGCTCTCTTCCGCCCTTTCCACAGAGTCGTACAAGG10.9615384615384616No Hit
CATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAT10.9615384615384616No Hit
GGTGTGCTTTGTTCATGGCTGCAGAACTAACAGATGATCTTCTTATTTTC10.9615384615384616No Hit
ATCCTAGGCCACGCACGCTGCTGCAGGGTCGAGGCTGTGTCTGCTTATTT10.9615384615384616No Hit
ATCCTGATGTGCGCGCCTGCGCCGTGCGCCGCATGCTCGAGCAGACTCCC10.9615384615384616No Hit
GAGTTTTGTGAAATTGAATTTTACCACAATTACAAGTTTTAAAATTTAGA10.9615384615384616No Hit
ATAATACTCCATAGTGTATATGGACCACATCTACTTCATCCATTCATCCG10.9615384615384616No Hit
CAAGGACACCTGCCCTCACTCAGGTGGACAGACAGGCCGCCTCTGCTCTC10.9615384615384616No Hit
AGTCGTTTTTATAAAATTAGTCACTAATTGAAATCCAAACAAAATGTTAT10.9615384615384616No Hit
GCTGAGAGCAGAATTATTACTGCGAGAATCGGAGGTGGCTGAGTTGGGCT10.9615384615384616No Hit
ACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCT10.9615384615384616No Hit
GAAAACAAGAAGATCATCTGTTAGTTCTGCAGCCATGAACAAAGCCCACC10.9615384615384616No Hit
GGTTGTATGTTACTAGAAATGTATACTTTTCGTTTGGATTATCCAGCTTG10.9615384615384616No Hit
GTTTGTTTAAGCAGGTATAAAAAATACACAACAGCCTTTGGACAGGGTGA10.9615384615384616No Hit
GCCACAGTGCGCGCCTGTCCCCTTCTGAACGGGGGTCTGGCCGCTTCGCA10.9615384615384616No Hit
CTTAGGGGGGCACTGATCTCATCTGTGAAATGGGAATAATGATGTCTACT10.9615384615384616No Hit
ACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCAC10.9615384615384616No Hit
GCCTACTTGTGATCTCTCTCTCTCTGTCAAATAAATAAATAAAGTCTTTA10.9615384615384616No Hit
AAGTGTCTCAGATGAACTACAGGTAACCAGAGTTCCTTTTATTCTTGGAT10.9615384615384616No Hit
GTATGGATAGAAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG10.9615384615384616No Hit
CTTTACCTCCCTCCCCCCTTGAAACACTGGGGTAAAAAACACATGACATA10.9615384615384616No Hit
GGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCA10.9615384615384616No Hit
ACCATATTCTACCCATCCACTTTCCAGCATTGGATGAAGAGCCTCCCCTC10.9615384615384616No Hit
TTCCAGCATGATGGAGGCAATGGTGTCTAGGGCCCGAATTGATGCACGGA10.9615384615384616No Hit
ATTTCCAGTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGT10.9615384615384616No Hit
GCAATGAATCGAGAAAACTACTACTCATTGTTCAAGCACTTAGGGACAAC10.9615384615384616No Hit
GTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGA10.9615384615384616No Hit
GTACAAGGAAGAGGGGCCGGGGAGGGTCTGAAATAACCACCATCACCCAG10.9615384615384616No Hit
GTATTATAACGTCCGTGGTTCCTGGAAATGCTGTGGGCATGTTTGCCTTT10.9615384615384616No Hit
TTCTATGGATGATTCGTAGAATTCACAGGTGAAACCATGGGGTCCTGAAC10.9615384615384616No Hit
CAAGTAGGCAGAGAGGCAGGCAGAGAGAGGAGGAAGCAGGTTCCCTGCTA10.9615384615384616No Hit
TCATTAGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGT10.9615384615384616No Hit
CCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTG10.9615384615384616No Hit
ATTGAAACAAATAAGTTCGCTGCAATATGCACACACTTAGAAGTCTGCTT10.9615384615384616No Hit
GTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGT10.9615384615384616No Hit
GTATTATTTTTTCACTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT10.9615384615384616No Hit
GAACATGTCGTTTCACAACTAGATTAATTAACATCACATATGATCTCCAA10.9615384615384616No Hit
AATGAAACAGAATTTGCCTGGCGGCCTTAGCGCGGTGGTCCCACCTGACC10.9615384615384616No Hit
TCTCAGGGTCCTGGGATCGAGCCCCACGTCGGGCTCTCTGCTCAGCGGAG10.9615384615384616No Hit
CTCCTAGTACGAGAAGACCGGAGTCGACGCATATCTGGAAAACGCGTTGT10.9615384615384616No Hit
GTCTTCAGGTAATGGTCCATCAATAGGGTTGAGTTGGGGTGCTCCGGTCT10.9615384615384616No Hit
GAGTAATCAGATTCCTAGAAACACAAAGTAGAATAGTGGTTGGAAGGGGC10.9615384615384616No Hit
CTCTTCGAGTGAGTCACAGCACTGAGCCACCTGGCTAGGGGCCTGGGATC10.9615384615384616No Hit
GAAGTAAACTGTAAATTCATAAACACAGAGACTAAACAACATTCTACTGA10.9615384615384616No Hit
GCGTGGTGCCTGCTACACACAATAGGTGTTTCATGGTAGTTTGGTAGGAT10.9615384615384616No Hit
GATTCTTGGTTTTGGCCCAAGTCATGATCTCAGGGTCATGAGATCAAGCC10.9615384615384616No Hit
ATTCTAAAAGCAAATATTCATAATATACTCCGAGGTGGGCAAACAGGCAG10.9615384615384616No Hit
TTCCCACATTCAGCCCTAGTGAGGGTCGTGATGACCTTAGATAGCTTCAC10.9615384615384616No Hit
GATTAGAATTTTCAACTAGGATGAATGTTGAGCATTGTCACCACTCAGAG10.9615384615384616No Hit
GCCTTCGGTTCAGGTCATGATCCCGGGGTCCTGGGGTCGAGCCCCGCATT10.9615384615384616No Hit
ACTTCTAATTGTGGTAAAATACAATAACACAAACCTCGCACCTTAATCAT10.9615384615384616No Hit
GTTCCTCATGACGGCTTCTCAGTTATGTGTAATCGTCATTTTGTCGTGAG10.9615384615384616No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers