FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2254_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2254_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1746468
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC47410.2714621739419216No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT40480.23178208819171037No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT32650.18694874455186125No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG29760.1704010608840242No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT26950.154311444584155No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG26510.15179207406033204No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT25410.1454936477507747No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT25100.14371863669989945No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT23550.1348435814455232No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG23460.1343282556565594No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA20020.1146313588339437No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA19860.11371522409800809No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC19570.11205472988912478No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA19570.11205472988912478No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC19480.11153940410016101No Hit
ATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTC19450.11136762883717309No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC18560.10627162936853124No Hit
ATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTCATGAAGCTTA18530.10609985410554329No Hit
ATTGTACTGCATTAATTCCATTATCAGTTGTAGTGCCCTTCATTGGCTCT18370.10518371936960769No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC17520.10031675358494975No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC17490.1001449783219618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG1051.6370905E-1162.270721
GCCGACC1400.056.95729145
GTTGACA12800.048.243591
AGTCCGC2400.042.28647145
ACATGGT14150.042.0125855
GCGAAAG2300.041.028442
ATCTATG9950.040.8879781
CCGACTA4150.040.1803934
GCGTTAA3700.039.1832549
TTACGTG2250.038.7140122
TACTGCG3800.038.1564875
TGCGTTA3800.038.152128
CAAGCGG800.002033394336.26323
GTATTAT24300.035.8761331
GTTACGT2451.8189894E-1235.5832671
AGCGAAA2451.8189894E-1235.5832671
CGAAAGC2700.034.9201163
ACGTGTT2501.8189894E-1234.7997134
GGTCCTA21250.034.112489
GTACTGC9950.033.517314
Produced by FastQC (version 0.11.5)