FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2254_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2254_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599106
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC45080.28190751582446694No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC29630.1852910313637745No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT25050.15665002820325857No Hit
ATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTC23410.14639429781390353No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT21870.13676391683853353No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC21530.13463772883098432No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA21080.13182365646805153No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA20830.13026028293308886No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG20780.12994760822609633No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC20780.12994760822609633No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG20560.1285718395153292No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG20270.1267583262147725No Hit
ATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTCATGAAGCTTA20070.12550762738680238No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA19730.12338143937925315No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC19570.12238088031687705No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA18990.11875385371576369No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA18570.1161273861770264No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT17180.10743502932263403No Hit
TTTCTATGTTCATTGGCTTTTGGTCCATCTTCCCTACTAAGCTGTAAGCT16620.10393307260431765No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC406.5910455E-572.6621551
TATTCCG651.3945464E-766.951063
GTTGACA15800.052.4271281
GGTTATA5450.051.9967771
GCTACTA4150.047.1633159
ACATGGT17700.046.2829485
GCGTTAA1351.027729E-842.9580849
CAGTGCA12100.041.4030042
GACATGG20750.039.482384
ATCTATG8900.039.188581
GGTCCTA26400.038.991789
TGACGCG750.001484253338.66348145
AACCGAT2109.094947E-1238.031332
AGCGAAA3250.038.0078961
CCGAGGT3450.037.826524
TGCGTTA1553.4760888E-837.4151048
GCGAAAG3550.036.8139422
AGTGCAG13700.036.5298653
ATATGTT7900.035.789315
TGGTCCT28850.035.680528
Produced by FastQC (version 0.11.5)