FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2254_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2254_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675204
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC33880.20224402520528842No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT28990.1730535504929549No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT21930.13090942953813386No Hit
GTGCTATGTGATCCATTCCCTCCTTCCTGAACTGGCAATCCAGATAAAGG19770.11801547751796199No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT19120.1141353530674473No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC18770.11204605528640094No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT17760.10601693883252428No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC1255.182301E-946.417833
TTACGTG1950.044.6672132
GTTACGT1903.6379788E-1242.0651931
AGCGAAA3500.039.4429441
GTTGACA10750.036.4981
CCGACTA3400.036.2509424
ACGATAA1406.6226494E-736.243366145
GCGAAAG4450.034.2532272
GCCGACC1501.1361226E-633.82714145
GTATACG1301.630954E-533.534491
TAGTCGG2257.9489837E-1032.2163288
ACATGGT12200.032.089085
ACGTGTT2859.094947E-1230.527114
TGTACGT1204.063559E-430.2028068
TTAGGTA6450.029.2255634
CGAAAGC4750.029.0111473
ACTACGC1050.007628407327.679261
GCGATTA1855.8274945E-627.42741145
GACGACT1658.420418E-526.3643194
GTTATGT9400.026.2805751
Produced by FastQC (version 0.11.5)