FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2254_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2254_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356960
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC21260.1566737413040915No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT18650.13743957080532956No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG16160.11908972998467163No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT15860.11687890578941162No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT15130.11149923358094564No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA352.7643364E-7103.55847145
GTCGTAA406.0590173E-790.774221
TTACGTG2000.065.316452
GTTACGT2050.063.7633551
ACGTGTT2350.055.5331574
TGACGCG450.00964463548.327293145
CGATACA752.5529553E-548.327293145
CGTAAAG803.7308055E-545.321873
AGCGAAA3000.043.5716251
TACGTGT3200.040.789683
GTATACG907.411631E-540.3440971
GCGAAAG3300.039.5857282
CCGACTA3500.037.2865454
GTTATGT8500.035.0281681
CGAAAGC3950.033.0448043
CAGTGCA8450.032.6367572
AAGTCGA1851.6207923E-731.4029751
TATACTG3950.031.2009325
GTTGACA11150.030.6108591
GTGCAGC9550.030.3671724
Produced by FastQC (version 0.11.5)