FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2254_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2254_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400656
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT24890.17770244799579626No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC19300.13779257719240126No Hit
GTGCTATGTGATCCATTCCCTCCTTCCTGAACTGGCAATCCAGATAAAGG18930.1351509578368993No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC18640.13308049942312744No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG17300.12351355364914726No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC16510.11787333934956193No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT15940.11380381763973452No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG15500.11066243246021863No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT14940.10666430586810752No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC14730.10516500839606584No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG451.1947787E-464.436226145
GTCGTCG553.221476E-452.72055145
TTACGTG1900.049.669862
GTTACGT2050.049.6050451
ACGTGTT2200.042.843094
TATGGAC4500.041.8865365
ACTACGC907.401786E-540.353311
AGCGAAA2700.040.353311
CTTATAT5400.039.00821
CAGTGCA7100.038.853352
GATAGCG4700.038.558914145
CGAAAGC3050.038.0523873
CCGAGGT4850.037.373044
TAATACG800.00203638436.2518464
GTTGACA12450.035.58871
GCGAAAG3350.034.671962
TTAGGTA4000.034.4392554
ACATGGT12450.034.35555
AGTGCAG8100.034.0298773
CATTCTA18000.033.474092