FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2254_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2254_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331264
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC25560.19199798086630449No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT20660.1551908562088361No Hit
GTGCTATGTGATCCATTCCCTCCTTCCTGAACTGGCAATCCAGATAAAGG19570.14700314888707275No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT14730.11064672371520601No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC14640.10997067448680353No Hit
ATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTC13730.1031350656218451No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGATT657.34294E-444.608624145
GTTACGT2005.456968E-1239.954491
GCCGACC1502.6040652E-838.66081145
GCTATAT1251.2482546E-534.8693771
TTACGTG2302.5465852E-1134.7090722
CGACAGC850.002742832334.116326
GGTTATA3400.032.049061
GACGACT1153.1626827E-431.5216984
AGCGAAA2807.2759576E-1231.1333711
CCGTACA950.00472788330.5262764
GTTGACA10500.030.4415191
CAGTGCA6200.030.4340532
ACGTGTT2701.72804E-1029.5374
GTATTAT12700.029.1722151
TTAGGTA5000.028.9999624
AGACGGT1255.15932E-428.995607145
CAGCGCG1000.006079670528.9956059
GTTCGAT2054.0461964E-728.2883999
ACGGTAG1306.4898335E-427.880399
AACGAAC1050.007709401727.6190134
Produced by FastQC (version 0.11.5)