FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2254_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2254_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603266
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT11050.18316961340436888No Hit
GTGCTATGTGATCCATTCCCTCCTTCCTGAACTGGCAATCCAGATAAAGG7650.12680973235687076No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC7370.12216833038825327No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC6930.11487469872328293No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC6800.11271976209499623No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT6800.11271976209499623No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT6660.1103990611106875No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG6460.10708377399024642No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC6410.10625495221013616No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG100.0070892484145.00635
GTTACGT950.076.439511
AGCGAAA1300.061.4455951
ACGTGTT1251.8189894E-1258.0073284
ACCGTAA450.0095758248.4116821
AACGGGT1557.366907E-1042.1125683
GTAACGG1953.6379788E-1240.9637341
AGTCCGC907.484302E-540.27285145
ACATGGT5050.040.1997685
GCGAAAG2005.456968E-1239.90322
CCGACGC1651.366061E-939.54062145
AAGGTCG750.001483810738.6619389
CGAAAGC2109.094947E-1237.9904373
GTTGACA5200.037.7052541
ACGGGTT1752.4228939E-937.2904244
CACGACT1001.3897613E-436.254584
CGGGTTG1803.1941454E-936.251575
GCTACTA800.002037198536.2455679
TAACGGG1854.147296E-935.295212
CAGTGCA3150.034.548222