FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2254_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2254_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402576
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC26270.18729822840259636No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT17850.127265830871197No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC14530.10359509930299676No Hit
GTGCTATGTGATCCATTCCCTCCTTCCTGAACTGGCAATCCAGATAAAGG14520.10352380191875521No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT14250.10159877254423288No Hit
ATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTC14090.10045801439636784No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC902.023262E-856.4840971
ACCGTAC400.00601113854.466811
CCGACGC3750.048.326916145
AGCGAAA3000.043.573451
GCCGACC1851.8189894E-1243.102386145
GCGTTAA1252.722827E-740.594619
CTGCCGC6050.039.540207145
AGTCGAA1303.6716665E-739.0764662
CGAAAGC3350.038.972283
GCGAAAG3450.037.8629072
GTTACGT1752.391971E-937.3486671
AAGGTCG1604.5973138E-836.245199
GTACTAG1051.8358046E-434.58211
AAGTCGA1501.1194606E-633.8904571
CTACGCA1501.1257271E-633.8662722
GTCGAAG1551.4605612E-632.756273
TGCGTTA1601.8797764E-631.714548
GTACTGC4850.031.3931794
ACGTGTT2351.2623786E-930.8524684
TACTGCG1652.3891407E-630.754595
Produced by FastQC (version 0.11.5)