FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2253_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2253_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029873
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC30420.14986159232621943No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG2353.45608E-1133.9323929
GCGAAAG6750.033.3278922
CGAAAGC6900.031.5283283
GCCGACC2451.9772415E-929.588543145
AGCGAAA7650.028.4836391
CCTATAC5600.027.1931823
ACCGTCG1358.090853E-426.8488648
GTGATCG2456.452319E-826.6296889
CTGCCGC7400.025.470139145
TACGCCG1450.00122714624.9990655
TGACGCG1450.001227674824.99722145
GTCCTAT22600.023.461191
GAATAAC17150.023.293471
CGGTTGA1600.002177324122.653728145
AATCGGC1600.002177324122.6537287
GTATACG1650.002574176822.0100841
CTATACT6400.021.5247544
TCCTATG23700.021.433822
TAGTACC10000.020.297749
GTTGACA13800.020.0004671
Produced by FastQC (version 0.11.5)