FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2253_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2253_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685157
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC30490.18093269647872573No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT17470.10366986577511768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC7300.041.726963
GCGAAAG7000.041.480042
TCTATAC17500.037.2987943
AGCGAAA8100.035.874661
GTTCTAT20400.034.1864431
TATACCA19750.031.9346395
CTATACC20450.030.4897354
GCCGACC4300.026.973392145
AATTCGC1358.0880884E-426.850116
TATACTG7800.026.0239525
GCCTACG1450.001212469925.050411
TACCACT23900.023.6581487
GTCCTAT15850.023.3751141
GAATAAC10550.022.7234541
ATACCAC26650.022.3062446
TAATACA9900.021.9702264
TATACGA1650.00260257221.9682735
TAGTACC4659.094947E-1221.8284844
TAATTCG2003.1056206E-421.7485895
TTCTATA28900.021.3500212
Produced by FastQC (version 0.11.5)