FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2253_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2253_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2436404
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC34150.14016558830144754No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT29370.1205465103488584No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC26510.108807898854213No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA24940.10236397576099858No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG24490.10051699143491802No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT2300.041.0617181
GTTGACA18150.038.4252051
ACCGTAC1354.8966103E-737.6691551
GTCTACG1051.8324552E-434.594121
GCGAAAG5600.032.400672
ACATGGT21300.031.993355
GTATTAT31450.030.9532131
TTACGTG3151.8189894E-1229.9526182
AGCGAAA5850.029.8041671
GCCGACC3001.8189894E-1128.995253145
GCGTTAA2502.4556357E-928.9946589
CTGCCGC8650.028.492441145
TATACTA5200.027.8829125
TACTGCG2654.5674824E-927.3568175
TGACATG29000.025.5083893
GTGATCG2859.929863E-925.4339089
GACATGG27850.024.4703874
GTACTAG3352.064553E-923.8544541
CGAAAGC7300.023.8434563
GGTCCTA34050.023.842899
Produced by FastQC (version 0.11.5)