FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2253_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2253_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908897
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC32110.16821232366125569No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT23780.1245745579777222No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC20450.10712992895897473No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACA18100.042.944321
GCTACTA4750.036.6274879
ACATGGT21300.036.4170765
GCAGTGC14150.034.9102551
CAGTGCA14800.032.8542862
GACATGG24000.032.6264884
GCGAAAG4500.032.2549552
AGCGAAA4750.030.5871
TCGACAT1902.0774314E-730.5277064
CGACTCC2651.382432E-1030.0909796
GGTCCTA31750.029.9100469
GGTTATA9400.029.3667761
TGACATG29200.028.8124963
ACGACTC2303.4940967E-828.3671445
CTGCCGC6400.028.317892145
TATACTG10350.028.016935
CGTACCC1050.007708465727.6203024
AGTGCAG17900.027.5525363
CGAAAGC5300.027.3690593
ATGGTCC35150.027.01697
Produced by FastQC (version 0.11.5)