FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2253_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2253_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429619
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG22010.15395710325618223No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC19810.13856838780122535No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG19020.13304243997876358No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT17610.12317967234626848No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA17510.12248018528013407No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT17250.12066151890818462No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT16860.11793351935026045No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA16570.11590500685847069No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA16560.11583505815185725No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC16350.11436613531297499No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA15170.10611218793258903No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT14930.10443341897386647No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1900.065.009391
GCGAAAG3300.048.3908652
AGCGAAA3400.047.013711
CAGTGCA8950.045.4171262
GTTGACA15450.041.854511
TTACGTG2950.041.829392
GCTACTA2750.039.5371369
AGTGCAG10850.038.1035353
CGAAAGC4200.037.9921233
ACATGGT17450.037.3899155
GATAGCG5200.034.84844145
ACGTGTT3550.034.717084
CCGACTA3800.034.340894
GGTGCGA1905.4405973E-934.334888
TGACATG24700.032.594643
CAGCAAC9650.032.3011637
CTACGCA900.003629378332.221084
TAGCATG20200.031.9810922
CAAGCGG1153.1550037E-431.5349623
CTTATAT5550.031.4194831
Produced by FastQC (version 0.11.5)