FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2253_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2253_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389510
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC4600.11809709635182666No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT4470.11475956971579677No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT4180.10731431798926858No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT3910.10038253189905266No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT300.001925168672.5917361
TTACGTG502.0025473E-458.0360762
GCTACTA805.875572E-754.3599329
ACGTGTT604.9387047E-448.3385544
ACATGGT3150.043.7348825
GTTGACA3200.043.1013451
GCGAAAG855.295795E-542.701021
CGAAAGC855.315918E-542.6735882
ATCTATG2100.041.4809881
CAGTGCA2300.041.003752
GCAGTGC2350.040.1571271
CAGCTAC1157.138775E-637.820467
GATATGT1604.526555E-836.2958681
GTCCGAG800.002022495736.2958681
ACTGTAC1001.3795869E-436.2958681
GCCGACC1001.3921582E-436.239952145
TACACAA2302.7284841E-1134.664303145
GACTGAC850.00274193934.112577
GACATGG4250.032.4152684
TACGTGT900.003623613232.2257043
Produced by FastQC (version 0.11.5)