FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2253_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2253_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1611116
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC31070.1928476906690766No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC24220.15033057830721067No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG23140.14362715037278506No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG21820.13543407178626493No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG21610.1341306274656822No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA18520.11495137531996455No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT17810.11054449214085144No Hit
TTAGTAGACAGCTGAACAGTCAGATGTCTGTTTCTTTAGAATTCAATCTT17570.10905484148875685No Hit
GATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTATG16820.10439968320096132No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT16730.10384106420642586No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA16650.103344513989061No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACT1550.065.482534
AGACGAC1900.057.255213
GGTTATA4250.049.5819171
ACGACTC2050.049.5096445
GTTACGT1056.788832E-848.442111
CGAATAT1650.048.32484145
CGAAGCG450.009646893548.3248379
AGTCCGC1002.7384976E-643.492355145
AAGACGA2350.043.2456362
ATCTATG7000.042.559851
GTTGACA14100.040.711981
GCGATTC907.485389E-540.2769474
AAGGTCG3650.039.7190489
GCCGACC1651.369699E-939.538506145
CTGCCGC6500.039.031605145
ACATGGT15150.037.3248065
GTACTGC4350.036.6659164
TTACGTG1406.548398E-736.2954442
GTAGAAA11850.035.529722
GAATAAC13650.035.1338351
Produced by FastQC (version 0.11.5)