FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2251_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2251_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006561
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC32580.16236735389554566No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5750.037.857022
CTGCCGC7800.033.456356145
GCGTAAC1102.4108264E-433.015261
CGAAAGC7300.030.795953
GTCTACG950.004686036630.5825581
AGCGAAA7150.030.4756221
GTTGACA16450.027.3755721
GTGATCG3204.1836756E-1127.1832929
GTCGTCC1400.001000714525.8888478
TATAATA12000.025.395752
GGTCCTA22450.023.8938489
TACTGCG2451.9141844E-623.6727525
GTATATA11700.022.969591
GTATAAT8300.022.7526841
TATTATT20850.022.6204032
GAATAAC15200.022.4590641
GTATTAT20350.021.4153041
ACTCCGA4109.1858965E-1021.2586041
ATGGTCC27100.020.329467
TTATTCG2505.985467E-520.296856145
Produced by FastQC (version 0.11.5)