FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2251_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2251_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1849356
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC47590.257332822885372No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT24510.1325326221668516No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG657.3432963E-444.6091045
GCCGACC2600.036.242935145
CTAGCGC850.002736483734.133153
TATACCG900.003631524332.2176865
GTTGACA15000.028.5667171
AGCGTAT1855.827238E-627.4278286
TCTAGCG1606.8101304E-527.199853
TAATGCG1358.091974E-426.8480725
GCGAAAG5650.025.7087611
GTCCTAT24450.025.5457821
CTGCCGC6250.025.51503145
GCGTATG2001.0677195E-525.3700567
TAGCGTA1801.5224199E-424.1632655
ACCGTAC1500.001496449724.1619578
TCTATAC38700.023.9902233
AGCGAAA5650.023.1378841
TCCTATG25100.023.1355932
GTTCTAT38800.022.4620381
GAATAAC15250.022.3834821
GTATACG1650.002575330422.0082571
Produced by FastQC (version 0.11.5)