FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2251_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2251_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2370621
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC30560.12891136963690106No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6600.042.9305081
GCGAAAG6950.041.7706152
CGAAAGC8850.032.7786873
GTATTAT26150.032.227871
CTAGTAC3100.030.4128063
TATACGG1753.778152E-628.9993465
CTGCCGC9550.025.807125145
GTTGACA12950.025.2457391
GTCCTAT21750.023.3821331
GTATAAG8300.022.758341
TAGTACC7350.022.6827359
GTATAAT7100.022.5117721
TATACTG9900.021.9692025
TAGTACT8100.021.4823574
CCGACTA3106.248956E-721.0492444
TATAATA21200.020.8828472
ACATGGT15800.020.1894195
ATACAGC16600.020.0886386
TACAGCC14250.019.838317
ATCTAAA27850.019.565071
Produced by FastQC (version 0.11.5)