FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2251_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2251_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549294
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC17690.11418103988010023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA651.5661499E-978.06737145
AGCGAAA3550.049.1079331
GCGAAAG4400.039.5827672
CGACCCG850.00274409534.11347145
GAATAAC8200.031.0043741
TATAATA9800.030.3602542
TATACTG5650.029.5125185
CGTTTAT1255.097732E-429.0555271
GATAGCG2507.845847E-826.096806145
CGAAAGC6750.025.7846073
TAGTACT5100.025.587584
AATACCG1450.001226930624.999365
GTAAGAC3203.3791366E-822.6622523
GTATATA7700.022.640671
TTATAAT11100.022.2497271
CTGCCGC6850.022.223555145
GTATTAT15250.021.9107231
TATAGGT4659.094947E-1221.8273984
ACAGCGC2404.3689914E-521.1432448
GTGTTAC6700.020.5990681
Produced by FastQC (version 0.11.5)